Pulsed field gel electrophoresis : a practical approach
Author(s)
Bibliographic Information
Pulsed field gel electrophoresis : a practical approach
（The practical approach series, 158）
IRL Press at Oxford University Press, c1995
- hbk.
- : pbk.
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Note
Includes bibliographical references and index
Description and Table of Contents
- Volume
-
: pbk. ISBN 9780199635351
Description
PFGE is the most reliable method for separating large fragments of DNA and has had a major impact on biological research. This book presents detailed chapters on both the theory behind PFGE amd protocols for most of its major research and diagnostic applications. This book is intended for researchers at all levels in labs involved in molecular human genetics, mammalian genetics (mouse, rat, etc), molecular microbiologists, molecular parasitologists, biochemists who use molecular biology techniques, and some molecular diagnostic labs.
Table of Contents
- 1. Theories of gel electrophoresis of high molecular weight DNA
- 2. PFGE in physical mapping
- 3. Mutation detection and diagnosis using PFGE
- 4. Cloning from PFGE-purified genomic DNA fragments and YACs
- 5. Yeast artificial chromosome cloning using PFGE
- 6. PFGE analysis of yeast artificial chromosomes
- 7. Functional analysis of mammalian genomes using yeast artificial chromosomes
- 8. PFGE in the study of a bacterial pathogen (Haemophilus influenzae)
- 9. Analysis of the genomes of protozoan parasites using PFGE
- Volume
-
hbk. ISBN 9780199635368
Description
Pulsed field gel electrophoresis (PFGE) is the most reliable method for separating large fragments of DNA and has had a significant impact on the analysis of both prokaryotic and eukaryotic genomes. This book presents detailed chapters on both the theory behind PFGE and protocols for most of its major research and diagnostic applications, providing an essential resource for laboratories requiring PFGE in their research strategies. This book is intended for researchers at all levels in labs involved in molecular human genetics, mammalian genetics (mouse, rat, etc.), molecular microbiologists, molecular parasitologists, biochemists who use molecular biology techniques, and some molecular diagnostic labs.
Table of Contents
1.: Theories of gel electrophoresis of high molecular weight DNA. 2.: PFGE in physical mapping. 3.: Mutation detection and diagnosis using PFGE. 4.: Cloning from PFGE-purified genomic DNA fragments and YACs. 5.: Yeast artificial chromosome cloning using PFGE. 6.: PFGE analysis of yeast artificial chromosomes. 7.: Functional analysis of mammalian genomes using yeast artificial chromosomes. 8.: PFGE in the study of a bacterial pathogen (Haemophilus influenzae). 9.: Analysis of the genomes of protozoan parasites using PFGE
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