Nitroxide spin labels : reactions in biology and chemistry
Author(s)
Bibliographic Information
Nitroxide spin labels : reactions in biology and chemistry
CRC Press, c1995
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Note
Includes bibliographical references (p. 211-263) and index
Description and Table of Contents
Description
Extending the use of nitroxides as probes into more complex systems such as viable cells and whole animals requires greater knowledge of their interactions in functioning biological systems and appropriate model systems. Nitroxide Spin Labels: Reactions in Biology and Chemistry presents information essential for research in this area. Nitroxide research offers the promise of important new ways of measuring metabolism and will be useful in the diagnosis of important disease categories such as cancer, inflammation, and ischemia.
Table of Contents
Introduction
General Considerations
Use of Nitroxides as Contrast Agents for Nuclear Magnetic Resonance Imaging and Spectroscopy
EPR Imaging and In Vivo EPR Techniques
Measurements of the Concentration of Oxygen
Measurement of Diffusion
Measurement of Viability
Measurement of Temperature
Summary and Plan of the Book
Terminology, Classification, and Distribution of the Nitroxides in Cells
General Analysis and Terminology
Classification
Distribution of Nitroxides in Cells
General Considerations
Distributions of Neutral Nitroxides
Location of Charged Nitroxides in Cells
Location of Doxyl Strearates and Related Lipophilic Nitroxides
Location of Phospholipids Labeled with Nitroxides
Chemical Reactivity of Nitroxides
General Aspects
Stability of Pure Solutions
Electrochemical Reactions
Reduction
Mechanisms of Chemical Reduction
Reduction of f-Phenylenediamine
Reduction by Metal Ions
Reduction of Nitroxides by Ascorbic Acid and Flavins
Reoxidation of Hydroxylamines and Amines to Nitroxides
Interconversion of Hydroxylamines and Nitroxides
Reduction of Nitroxides by SH-Containing Substances
Reactions of Free Radicals with Nitroxides
Photochemical Reactions
Nitroxides as Redox Probes of Melanin
Interactions with Singlet Oxygen
Radiation-Induced Reactions
Reduction Reactions
Oxidation Reactions
Radiosensitization by Nitroxides
Inhibition of Lipid Peroxidation
Reactions of Superoxide with Nitroxides
Nitroxides as Catalysts of Radical Reactions
Spin Trapping
Challenges for Chemists and Biochemists
Applications of Reactions of Nitroxides in Biophysics
General Considerations
The Kinetics and Mechanism of Reduction of Nitroxides by Ascorbic Acid
Investigations of Model and Biological Membranes
Transport of Ascorbate
The Kinetics of Reductions in Membranes
Reduction of Spin-Labeled Lipids
Dynamics of Lipids
Membrane Permeability an
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