CiNii 図書 - Biochemistry, molecular biology, and drug discovery

AIDS is one of the most pressing medical emergencies of our era. Despite arguments to the contrary, the majority of AIDS researchers have agreed that there is a causal link between HIV infections and AIDS. However, it is now 12 years since the discovery of HIV and we are still a long way from understanding and controlling AIDS. HIV 1 and HIV 2 are guides to studying HIV in the laboratory. HIV 1 describes studies of the virus and infected cells and covers techniques for handling HIV safely, for studying viral entry, replication, and neutralization, and basic immunological techniques. HIV 2 explores the biochemistry and molecular biology of HIV. Methods for cloning and expressing most of the viral proteins are described, plus assays for studying enzyme function in vitro. It also includes a section on recent approaches to drug discovery. HIV research is highly multidisciplinary so a complete compendium of all related methods would have been unreadable. Instead, theses two volumes focus on the specialized techniques which are unique to studies of HIV. The books have been designed so that a novice entering HIV research can find clear instructions about how to perform standard experiments, such as assays for reverse transcriptase or antibodies to HIV. In addition the books include many novel methods which are described in detail for the first time.

Section 1: Biochemistry and molecular biology of viral proteins
1. The HIV life cycle
2. Reverse transcriptase
3. Ribonuclease H
4. Integrase
5. Protease
6. Expression of gag, envelope and other HIV antigens using Bacculovirus
7. Envelope expression and purification
8. HIV envelope expression and purification
9. RNA binding assays for the regulatory proteins tat and rev
10. Cellular and cell-free assays for tat
11. Cellular assays for rev
12. DNA binding proteins interacting with the viral LTR
13. Nef
14. Vpu
15. Vif
SECTION 2: DRUG DISCOVERY
16. Cellular assays for antiviral drugs
17. Inhibition of HIV by ribozymes and antisense oligonucleotides
18. Gene therapy approaches to the inhibition of HIV

巻冊次: : hbk. ISBN 9780199634996

内容説明

HIV Volume 2 explores the biochemistry and molecular biology of HIV. Methods for cloning and expressing most of the viral proteins are described as well as assays for studying enzyme function in vitro. It also includes a section on recent approaches to drug discovery. Contents: The HIV life cycle; Reverse transcriptase; Ribonulease H; Integrase; Protease; Expression of gag, envelope and other HIV antigens using Bacculovirus; Envelope expression and purification; HIV envelope glyocoprotein/CD4 interactions - studies using recombinant vaccinia virus vectors; RNA binding assays for the regulatory proteins tat and rev; Cellular and cell-free assays for tat; Cellular assays for rev; DNA binding proteins interacting with the viral LTR; Nef; Vpu; Vif; Cellular assays for antiviral drugs; Inhibition of HIV by ribozymes and antisense oligonucleotides; Gene therapy approaches to the inhibition of HIV This book is intended for these books provide instructions in virological, biochemical, molecular biological, and immunological methods of use for HIV researchers in academic institutions, medical schools, and the pharmaceutical industry.

Section 1: Biochemistry and molecular biology of viral proteins. 1.: The HIV life cycle. 2.: Reverse transcriptase. 3.: Ribonuclease H. 4.: Integrase. 5.: Protease. 6.: Expression of gag, envelope and other HIV antigens using Bacculovirus. 7.: Envelope expression and purification. 8.: HIV envelope expression and purification. 9.: RNA binding assays for the regulatory proteins tat and rev. 10.: Cellular and cell-free assays for tat. 11.: Cellular assays for rev. 12.: DNA binding proteins interacting with the viral LTR. 13.: Nef. 14.: Vpu. 15.: Vif. Section 2: Drug discovery. 16.: Cellular assays for antiviral drugs. 17.: Inhibition of HIV by ribozymes and antisense oligonucleotides. 18.: Gene therapy approaches to the inhibition of HIV

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Biochemistry, molecular biology, and drug discovery

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