Biochemistry, molecular biology, and drug discovery
著者
書誌事項
Biochemistry, molecular biology, and drug discovery
（The practical approach series, v. 157 . HIV : a practical approach ; v. 2）
IRL Press at Oxford University Press, c1995
1st ed
- : hbk.
- : pbk.
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk. ISBN 9780199634989
内容説明
AIDS is one of the most pressing medical emergencies of our era. Despite arguments to the contrary, the majority of AIDS researchers have agreed that there is a causal link between HIV infections and AIDS. However, it is now 12 years since the discovery of HIV and we are still a long way from understanding and controlling AIDS. HIV 1 and HIV 2 are guides to studying HIV in the laboratory. HIV 1 describes studies of the virus and infected cells and covers
techniques for handling HIV safely, for studying viral entry, replication, and neutralization, and basic immunological techniques. HIV 2 explores the biochemistry and molecular biology of HIV. Methods for cloning and expressing most of the viral proteins are described, plus assays for studying enzyme
function in vitro. It also includes a section on recent approaches to drug discovery.
HIV research is highly multidisciplinary so a complete compendium of all related methods would have been unreadable. Instead, theses two volumes focus on the specialized techniques which are unique to studies of HIV. The books have been designed so that a novice entering HIV research can find clear instructions about how to perform standard experiments, such as assays for reverse transcriptase or antibodies to HIV. In addition the books include many novel methods which are described in
detail for the first time.
目次
- Section 1: Biochemistry and molecular biology of viral proteins
- 1. The HIV life cycle
- 2. Reverse transcriptase
- 3. Ribonuclease H
- 4. Integrase
- 5. Protease
- 6. Expression of gag, envelope and other HIV antigens using Bacculovirus
- 7. Envelope expression and purification
- 8. HIV envelope expression and purification
- 9. RNA binding assays for the regulatory proteins tat and rev
- 10. Cellular and cell-free assays for tat
- 11. Cellular assays for rev
- 12. DNA binding proteins interacting with the viral LTR
- 13. Nef
- 14. Vpu
- 15. Vif
- SECTION 2: DRUG DISCOVERY
- 16. Cellular assays for antiviral drugs
- 17. Inhibition of HIV by ribozymes and antisense oligonucleotides
- 18. Gene therapy approaches to the inhibition of HIV
- 巻冊次
-
: hbk. ISBN 9780199634996
内容説明
HIV Volume 2 explores the biochemistry and molecular biology of HIV. Methods for cloning and expressing most of the viral proteins are described as well as assays for studying enzyme function in vitro. It also includes a section on recent approaches to drug discovery. Contents: The HIV life cycle; Reverse transcriptase; Ribonulease H; Integrase; Protease; Expression of gag, envelope and other HIV antigens using Bacculovirus; Envelope expression and purification; HIV envelope glyocoprotein/CD4 interactions - studies using recombinant vaccinia virus vectors; RNA binding assays for the regulatory proteins tat and rev; Cellular and cell-free assays for tat; Cellular assays for rev; DNA binding proteins interacting with the viral LTR; Nef; Vpu; Vif; Cellular assays for antiviral drugs; Inhibition of HIV by ribozymes and antisense oligonucleotides; Gene therapy approaches to the inhibition of HIV This book is intended for these books provide instructions in virological, biochemical, molecular biological, and immunological methods of use for HIV researchers in academic institutions, medical schools, and the pharmaceutical industry.
目次
Section 1: Biochemistry and molecular biology of viral proteins. 1.: The HIV life cycle. 2.: Reverse transcriptase. 3.: Ribonuclease H. 4.: Integrase. 5.: Protease. 6.: Expression of gag, envelope and other HIV antigens using Bacculovirus. 7.: Envelope expression and purification. 8.: HIV envelope expression and purification. 9.: RNA binding assays for the regulatory proteins tat and rev. 10.: Cellular and cell-free assays for tat. 11.: Cellular assays for rev. 12.: DNA binding proteins interacting with the viral LTR. 13.: Nef. 14.: Vpu. 15.: Vif. Section 2: Drug discovery. 16.: Cellular assays for antiviral drugs. 17.: Inhibition of HIV by ribozymes and antisense oligonucleotides. 18.: Gene therapy approaches to the inhibition of HIV
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