Protocols in human molecular genetics
著者
書誌事項
Protocols in human molecular genetics
（Methods in molecular biology / John M. Walker, series editor, 9）
Humana Press, c1991
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Protocols in Human Molecular Genetics highlights the tremendous advances in our ability to work on the human genome that have emerged in the past few years. The latest techniques are set forth in the clear, concise, easy-to-follow format that is the hallmark of Humana's Methods in Molecular Biology series. Nearly two-thirds of the book is devoted to describing practical procedures comprising the widest range of new methodologies in human molecular genetics, with the rest focusing on their specific experimental and clinical applications. An essential tool for everyone - whether novice or seasoned expert - involved in the rapidly growing area of human genome studies.
目次
The Polymerase Chain Reaction: Getting Started. Direct DNA Sequencing of Complementary DNA Amplified by the Polymerase Chain Reaction. Direct-Sequencing of PCR-Amplified DNA. Rapid DNA Sequence Analysis Using Fluorescent Labels. Detection of Mutations in DNA and RNA by Chemical Cleavage. Rapid Methods for Detection of Polymorphic Markers in Genomic DNA. The Analysis of Point Mutations Using Synthetic Oligonucleotide Probes. Detection of Mutations by the Amplification Refractory Mutation System (ARMS). Automated Gene Detection Using the Oligonucleotide Ligation Assay. Detection of Point Mutations by Denaturing-Gradient Gel Electrophoresis. The Detection and Mapping of Point Mutations by RNase A Cleavage. Discontinuous Polyacrylamide Gel Electrophoresis of DNA Fragments. Extraction and Enzymatic Amplification of DNA from Paraffin-Embedded Specimens. The Use of the Polymerase Chain Reaction in the Mapping of Human Genes Using Somatic Cell Hybrids. The Southern Blot: An Update. The Detection of Specific DNA Sequences by Enhanced Chemiluminescence. Pulsed-Field Gel Electrophoresis. Cloning from Gels Following Pulse-Field Gel Electrophoresis. Yeast Artificial-Chromosome (YAC) Cloning Systems. Gene Targeting for Somatic Cell Manipulation. In Situ Hybridization of Chromosomes. DNA Fingerprinting Analysis: Methodology and Its Applications. DNA Fingerprinting and Forensic Medicine. The Detection of Point Mutations in Hemoglobin Defects Using Allele-Specific Oligonucleotide Probes. Detection of Gene Deletions Using Multiplex Polymerase Chain Reactions. Application of Pulsed-Field Gel Electrophoresis to Genetic Diagnosis. Molecular Diagnostics of Cancer. The Detection of Latent Virus Infection by Polymerase Chain Reaction. Mapping Inherited Diseases by Linkage Analysis. Diagnosis of Genetic Disorders with Linked DNA Markers. Software for Genetic Linkage Analysis. Creating Animal Models of Genetic Diseases. Molecular Biology and Medicine: Ethical Implications. Appendix. Index.
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