Molecular microbial ecology manual
著者
書誌事項
Molecular microbial ecology manual
Kluwer Academic Publishers, c1995
- : hard
大学図書館所蔵 全13件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
注記
Includes bibliographical references
内容説明・目次
内容説明
This manual provides general information about molecular techniques relevant to molecular ecological research. Attention is paid to the use of molecular methods for studying the fate of genetically modified and native microorganisms in the environment. Postgraduate students and research scientists in the plant and soils sciences and plant molecular biology should find this book of interest.
目次
Section 1: Isolation of Microbial Nucleic Acids. 1.1: Extraction of Microbial DNA from Aquatic Sources. 1.2: Extraction of Microbial RNA from Aquatic Sources. 1.3: Extraction of Microbial RNA from Bulk Soil. 1.4: Extraction of DNA from Phytosphere, Rhizosphere, Rhizoplane. 1.5: Extraction of RNA from Bulk Soil. 1.6: Nucleic Acid Extraction from Cultures. Section 2: Detection of Microbial Nucleic Acid Sequences. 2.2: Preparation of Radioactive Probes. 2.3: Preparation of Non-Radioactive Probes. 2.6: Detection of Microbial DNA Sequences by Colony Hybridization. 2.7: Detection and Quantification of Microbial Nucleic Acid Sequence Polymerase Chain Reaction (PCR). Section 3: Identification and Classification of Microbes Using DNA and RNA Sequences. 3.1: Partial and Complete 16S rDNA Sequences, Their Use in Generation of 16S rDNA Phylogenetic Trees and Their Implications in Molecular Ecological Studies. 3.3: Microbial identification and Design of Phylogenetic Trees Based on rRNA Sequences. Section 4: Detection, Identification and Classification of Microbes Using Other Methods. 4.1: Detection, Identification and Classification of Microbes Using Other Methods. Section 5: Detection of Gene Transfer in the Environment. 5.1: Gene Transfer by Transformation. 5.2: Gene Transfer by Conjugation. 5.3: Gene Transfer by Transduction. Section 6: Tracking of Specific Microbes in the Environment. 6.1: Marker Genes. 6.2: Designing Field and Microcosm Experiments with GEM's.
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