Gel electrophoresis : nucleic acids

著者

    • Jones, P. (Paul)

書誌事項

Gel electrophoresis : nucleic acids

P. Jones

（Essential techniques series）

Wiley, 1995

大学図書館所蔵 件 / 全9件

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Gel electrophoresis of nucleic acids is the one technique that spans the whole range of molecular biology techniques. The combination of its high resolution and versatility of its applications makes it the one method used by all molecular biologists. This book gives clear, step-by-step protocols for all the important techniques from simple analytical separations of nucleic acids to the latest PCR techniques. Hence it will be essential reading for all those working in the area of molecular biology. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.

目次

  • INTRODUCTORY MATERIAL: Properties of Nucleic Acids and Nucleoproteins
  • Methods for Labelling Nucleic Acids
  • Types of Electrophoretic Separation
  • Types of Gels
  • Methods of Analyzing the Interaction of Nucleic Acids and Proteins
  • METHODS FOR LABELLING AND DETECTING NUCLEIC ACIDS: Radiolabelling
  • Non-Isotopic Detection Methods
  • GEL ELECTROPHORESIS OF RNA: Electrophoresis Using Agarose Gels in a Submarine Apparatus
  • Electrophoresis Using Polyacrylamide Gels
  • RNA Fingerprinting
  • Gel Retardation
  • DNA PROTOCOLS: Separation of Restriction Fragments
  • Analysis of PCR Products
  • Southern Blotting
  • Pulsed Field Electrophoresis (PFGE) of DNA
  • NUCLEOPROTEINS: Electrophoresis of Ribosomes
  • APPENDIX: Safety
  • Suppliers.

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