CiNii 図書 - Mammalian systems

DNA cloning techniques have made their impact to most areas of biological research. This is the fourth and last volume in this set, offering a complete update and extension into new areas of David Glover's original DNA Cloning volumes. This volume describes a variety of techniques for genetic manipulation of mammalian cells. Topics covered include construction of Vaccinia virus recombinants, retroviral vectors, production of transgenic animals, expression using the HSV-1 vector system, and adenovirus vectors. The information presented is an up-to-date account of each research area by established researchers. The book includes detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments. This new 4-part companion volume set of Practical Approach titles aims to cover the major techniques required by all modern molecular biology laboratories.

1. High efficiency gene transfer into mammalian cells
2. Construction and characterization of Vaccinia virus recombinants
3. Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells
4. Retroviral vectors
5. Genetic manipulation of embryonic stem cells
6. Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs
7. Genomic and expression analysis of transgenic animals
8. Expression using a defective Herpes simplex virus (HSV-1) vector system
9. Adenovirus vectors

巻冊次: : hbk ISBN 9780199634811

内容説明

This is the fourth and last volume in this set. This volume describes a variety of tecnhiques for genetic manipulation of mammalian cells. Topics covered include construction of Vaccinia virus recombinants, retroviral vectors, production of transgenic animals, expression using the HSV-1 vector system, and adenovirus vectors. This book is intended for phD students, research assistants, postdocs, academic staff, in the general areas of molecular biology, genetics, biochemistry, and medical research.

1.: High efficiency gene transfer into mammalian cells. 2.: Construction and characterization of Vaccinia virus recombinants. 3.: Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells. 4.: Retroviral vectors. 5.: Genetic manipulation of embryonic stem cells. 6.: Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs. 7.: Genomic and expression analysis of transgenic animals. 8.: Expression using a defective Herpes simplex virus (HSV-1) vector system. 9.: Adenovirus vectors

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Mammalian systems

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