Planar lipid bilayers : methods and applications
著者
書誌事項
Planar lipid bilayers : methods and applications
（Biological techniques series）
Academic Press, c1993
- : hard
- : bench-top
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注記
"Key references": p. [129]-130
Includes bibliographical references and index
収録内容
- Introduction
- Physical properties of biological membranes and planar lipid bilayers
- Technical details of bilayer experiments
- Biochemical preparations for planar lipid bilayer experiments
- Methods for making planar lipid bilayers
- Incorporation of proteins into planar lipid bilayers
- Data acquisition and evaluation
- Advanced experiments and examples of data
内容説明・目次
- 巻冊次
-
: hard ISBN 9780123229946
内容説明
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.
In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.
The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.
Methods of constructing artificial membranes (planar lipid bilayers) from the main components of cell membranes (lipids) date from the early 1960s.
Planar bilayers offer direct, quantitative experimental approaches to the study of membranes of precisely determined composition which can be manipulated by the experimenter. Pore-forming molecules, transporter molecules, ATP-dependent enzymes and other entities can be incorporated into the bilayers to simulate biological functions.
Reconstitution of such functions in this way remains a key final step in attributing a functional role to purified cell membrane proteins.
This book aims to demystify these techniques and begins with a broad overview of the development of the subject before dealing with the protocols involved.
Key references are provided at the end of the book together with a list of suppliers.
目次
Introduction: Planar Lipid Bilayers: Tools for Studying the Properties of Biological Membranes. Physical Properties of Biological Membranes and Planar Lipid Bilayers. Technical Details of Bilayer Experiments. Biochemical Preparations for Planar Lipid Bilayer Experiments. Methods for Making Planar Lipid Bilayers. Incorporation of Proteins Into Planar Lipid Bilayers. Data Acquisition and Evaluation. Advanced Experiments and Examples of Data. Appendices. Index.
- 巻冊次
-
: bench-top ISBN 9780123229953
内容説明
This bench-top manual provides help for beginners through to experienced scientists on setting up bilayer experiments. Cell membranes consist of lipid bilayers in which are contained many other entities (eg proteins forming ion charmels) that regulate cellular processes. Scientists are guided through the know-how on making simple artificial lipid protein systems in such bilayers. Technical tips on all the pitfalls and how to build your own set-up for experimental work round off the book.
目次
Physical Properties of Biological Membranes and Planar Lipid Bilayers. Technical Details of Bilayer Experiments. Biochemical Preparations for Planar Lipid Bilayer Experiments. Methods for Making Planar Lipid Bilayers. Incorporation of Proteins into Planar Lipid Bilayers. Data Acquisition and Evaluation. How to Control the Physical Properties of Planar Lipid Bilayers. Appendix 1. List of Symbols and Abbreviations used. Appendix 2. Letter Code for Amino Acids. Appendix 3. List of Useful Constants, Data and Equations. Appendix 4. Sources of Supply. Appendix 5. Key Reference.
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