Immunochemical approaches to coastal, estuarine, and oceanographic questions
著者
書誌事項
Immunochemical approaches to coastal, estuarine, and oceanographic questions
（Lecture notes on coastal and estuarine studies, 25）
Springer-Verlag, c1988
- : New York
- : Berlin
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注記
Papers presented at a workshop held at the Univ. of Southern Maine, Portland, Oct. 5-7, 1986
Includes bibliographical references and index
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内容説明・目次
内容説明
This volume represents a collection of pioneering papers introducing immunochemical techniques to the aquatic sciences. It is the product of a workshop entitled "Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions" held at the University of Southern Maine, Portland, Oct. 5-7, 1986. Funding from many sources made this workshop possible, and is gratefully acknowledged. The reader will note great variability in both the quality and scope of the papers which we have divided 'into four sections: I. Background material on the immune system (borrowed from a recent NIH publication) II. Background material related to aquatic sciences III. Techniques IV. Applications Readers familiar with terminology are directed to start with the original contributions related to aquatic sciences, page 21. Some of the contributions are abstracts only. Others are preliminary, for which we are grateful to the authors for their willingness to share recent sometimes tentative findings with a broader community. Some offer novel and tempting insights, but as they exist, lack adequate standards and controls. Others are thorough and represent major scientific contributions. The promise rests in generating and working with reagents of great specificity enabling orders of magnitude improvements in the sensitivity of our measurements. Authors also address the problems and limitations. We thank the numerous authors and reviewers. In support of every successful endeavor is someone of unusual savvy. For us, this has been David A. Phinney. His leadership, wisdom and wit have been important to the introduction of flow cytometry/sorting and immunochemistry into aquatic research.
目次
Background of the Immune System.- Understanding the Immune System.- Background.- What Does the Immunological Approach Offer Aquatic Research? An Overview.- Molecular Biology, Single Cell Analysis and Quantitative Genetics: New Evolutionary Genetic Approaches in Phytoplankton Ecology.- Techniques.- The Production of Monospecific Antisera to Soft-Bottom Benthic Taxa.- Preparation and use of Monospecific Antisera to Capitella SP. I.- Cell Affinity Chromatography for a Marine Nitrifying Bacterium.- Application of Immunoblotting for Dietary Analysis.- Two-Dimensional Electrophoresis with Silver Staining: A Sensitive Method for Analyzing Complex Protein Mixtures.- Brevetoxins and Binding: Sodium Channels Versus Antibodies.- Preparation of Model Haptens to Express Common Epitopes of PSP Toxins.- Monoclonal Antibodies (Monoabs) to Ciguatoxin and Related Polyethers.- Immunofluorescent Reagents: Preparation and Analysis.- Flow Cytometry and Immunofluorescence in Aquatic Sciences.- Detection Schemes for Antigen-Antibody Reactions.- Applications.- Identification of Marine Chroococcoid Cyanobacteria by Immunofluorescence.- An Immunofluorescence Approach toward the Identification of Endosymbiotic Diatoms in Several Species of Larger Foraminifera.- Immunofluorescent characterization of the Eukaryotic Ultraplankton.- Probes for Assessing Single-Cell Primary Production: Antibodies Againstribulose-1, 5-Bisphosphatecarboxylase (RuBPCASE) and Peridinin/Chlorophyll a Protein (PCP).- Examining Nitrate Reduction by Phytoplankton with an Immunoassay.- A Possible immunological Approach to The Study of Plasmalemma Redox Enzymes in Phytoplankton.- Antiluciferase Immunogold Labeling of two Different Organelles in the Marine Dinoflagellate Gonyaulax Polyedra.- Enzyme-Linked Immunosorbent Assay for Metal-Binding Proteins of Mytilus Edulis.- Environmental induction of Teleost cytochrome P-450 Detected by Immunochemical Analysis.- Quantifying Stomach Contents Using Immunoassays: A Critique.- Euphausiid predation on Larval Anchovy at two Contrasting Sites off California Determined with an Elispot immunoassay.- Enzyme immunoassay Utilizing Monoclonal Antibodies for identification of European Eel Virus (EEV), an Aquatic Birnavirus.- Monoclonal Antibodies to Atlantic Salmon (Salmo Salar) Immunoglobulin: Production And Preliminary Characterization.- Immunochemical evaluation of The infectious Pancreatic Necrosis (IPN) Carrier State in Salmonids and Other species.- References.
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