Methods in avian embryology
著者
書誌事項
Methods in avian embryology
（Methods in cell biology / edited by David M. Prescott, v. 51）
Academic Press, c1996
- : pbk.
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注記
Includes index
内容説明・目次
- 巻冊次
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: pbk. ISBN 9780121352752
内容説明
This volume presents a combination of classical embryological techniques and modern molecular biological approaches to studying the developing avian embryo. It also describes how to use these methods to study problems in cell, developmental and neurobiology. The protocols emphasize microsurgery, histology, and cellular and molecular marking. The methods include: embryonic transplantations, cell culture and organ culture, in situ hybridization, classical histological techniques, and retrovirally mediated gene transfer.
目次
Culture and Microsurgical Manipulation of the Early Avian Embryo. Quail-Chick Transplantations. Manipulations of Neural Crest Cells of Their Migratory Pathways. Manipulation of the Avian Segmental Plat in Vivo. Somite Strips: An Embryo Fillet Preparation. Embryo Slices. Operations on Limb Buds of Avian Embryos. Cell Marking and Image Analysis. Gene Transfer Using Replication-Defective Retrovital and Adenoviral Vectors. Manipulating Gene Expression with Replication Competent Retroviruses. Hybridization Analysis of Chick Embryos in Whole Mount and Tissue Sections. Micromass Cultures of Limb and Other Mesenchyme. Organ Cultures and Chorioallantoic Grafts. Autonomic and Sensory Neuron Cultures. Retinal Cultures. Migration and Adhesion Assays. Classical Histology and Cell Birthdating. Time Lapse Cinematography.
- 巻冊次
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ISBN 9780125641531
内容説明
A current and authoritative guide, Methods in Avian Embryology presents a combination of classical embryological techniques and modern molecular biological approaches to studying the developing avian embryo. The only one of its kind, this book is specifically devoted to providing a detailed approach to studying avian embryos. It also describes how to use this system to study problems in cell, developmental, and neurobiology. The protocols emphasize microsurgery, histology, and cellular and molecular marking, which are not covered in the usual molecular biology methods manuals. The methods include: embryonic transplantations, cell culture and organ culture, in situ hybridization, classical histological techniques, and retrovirally mediated gene transfer.
目次
M.A.J. Selleck, Culture and Microsurgical Manipulation of the Early Avian Embryo.
N. Le Douarin, F. Dieterlen-Lievre, and M.-A. Teillet,Quail-Chick Transplantations.
M. Bronner-Fraser, Manipulations of Neural Crest Cells or Their Migratory Pathways.
B.A. Williams and C.P. Ordahl, Manipulation of the Avian Segmental Plate in Vivo.
K.W. Tosney, R.A. Oakley, M. Champion, L. Bodley, R. Sexton, and K.B. Hotary, Somite Strips: An Empryo Fillet Preparation.
K.B. Hotary, L.T. Landmesser, and K.W. Tosney, Embryo Slices.
J.W. Saunders, Jr., Operations on Limb Buds of Avian Embryos.
S.E. Fraser, Iontophoretic Dye Labeling of Embryonic Cells.
S.M. Leber, M. Yamagata, and J.R. Sanes, GeneTransfer Using Replication-Defective Retroviral and Adenoviral Vectors.
B.A. Morgan and D.M. Fekete, Manipulating Gene Expression with Replication-Competent Retroviruses.
M.A. Nieto, K. Patel, and D.G. Wilkinson, In Situ Hybridization Analysis of Chick Embryos in Whole Mount and Tissue Sections.
K. Daniels, R. Reiter, and M. Solursh, Micromass Cultures of Limb and Other Mesenchyme.
M. Ferguson, Organ Cultures and Chorioallantoic Grafts.
R. Nishi, Autonomic and Sensory Neuron Cultures.
D. Finlay, G. Wilkinson, R. Kypta, I. de Curtis, and L. Reichardt, Retinal Cultures.
T. Lallier, Migration and Adhesion Assays.
J. Sechrist and C. Marcelle, Cell Division and Differentiation in Avian Embryos: Techniques for Study of Early Neurogenesis and Myogenesis.
H. Bortier, M. Callebaut, and L.C.A. Vakaet, Time-Lapse Cinephotomicrography, Videography, and Videomicrography of the Avian Blastoderm.
Subject Index.
Volumes in Series.
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