DNA isolation and sequencing
著者
書誌事項
DNA isolation and sequencing
（Essential techniques series）
John Wiley & Sons, 1996
- :alk. paper
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This convenient book provides immediate information on how to perform DNA sequencing and isolation techniques. It presents step--by--step instructions for 40 of the most commonly used methods in the field.
目次
Partial table of contents: GENERAL METHODS. Restriction Digestion. Agarose Gel Electrophoresis. Bacterial Cell Maintenance and Storage. RANDOM SUBCLONE GENERATION. Sonication. DNA Ligation. METHODS FOR DNA ISOLATION. Large Scale Double--Stranded DNA Isolation. Miniprep Double--Stranded DNA Isolation. METHODS FOR DNA SEQUENCING. Radiolabeled Sequencing Gel Preparation, Loading and Electrophoresis. Flourescent DNA Sequencing. ADDITIONAL METHODS. Polymerase Chain Reaction (PCR). Purification of PCR Fragments for Cloning. Synthesis and Purification of Oligonucleotides. Appendices. Index.
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