CiNii 図書 - Neural cell culture : a practical approach

This book brings together methods for preparing and maintaining cell cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems. This book is intended for research scientists in basic and clinical neuroscience/neurology (inc. pharmaceutical company R&D). Post graduate neuroscience/neurology, cell biology.

PART I - CNS NEURONS
1. Cerebellum: granule cells
2. Cerebellar Purkinje neurons
3. Nigral & straital neurons
4. Septal neurons
5. Proliferation & differentiation in retinal cell cultures
6. Purified embryonic motoneurones
PART II - CNS GLIA AND NON-NEURONAL CELLS
7. Astrocytes
8. Oligodendrocyte lineage cells from neonatal rat brain
9. Micrglia: the tissue macrophage of the CNS
10. Brain endothelium
PART III - PNS NEURONS AND GLIA
11. Neural crest cells: strategies to generate lineage diversifaction in vitro
13. Avian cilliary and lumbar sympathetic ganglion neurons
14. Enteric ganglion cells
15. Mouse olfactory epithelium
16. Schwann cell culture

巻冊次: : hbk ISBN 9780199634859

内容説明

The complexity and cellular heterogeneity of the vertebrate nervous system makes it difficult to study neural processes at the cellular and molecular levels. The establishment of cell culture model systems for almost every region and cell type in the nervous system has therefore been crucial in facilitating such studies and in heralding some key breakthroughs in our understanding of the underlying mechanisms. Neural Cell Culture: A Practical Approach brings together methods for preparing cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems of mammalian and avain species. Important features of this volume are the clearly illustrated dissection guides accompanying each chapter, methods for enriching and purifying distinct cell types, and guidance on optimizing cell viability and growth. Each chapter includes valuable step-by-step protocols, hints, and tips for success, advice, and background information. This book is intended for research scientists in basic and clinical neuroscience and neurology. Pharmaceutical company research and development staff. Post graduates of neuroscience, neurology, and cell biology.

Part I - CNS Neurons. 1: Cerebellum: granule cells. 2: Cerebellar Purkinje neurons. 3: Nigral & straital neurons. 4: Septal neurons. 5: Proliferation & differentiation in retinal cell cultures. 6: Purified embryonic motoneurones. Part II - CNS glia and non-neuronal cells. 7: Astrocytes. 8: Oligodendrocyte lineage cells from neonatal rat brain. 9: Micrglia: the tissue macrophage of the CNS. 10: Brain endothelium. Part III - PNS neurons and glia. 11: Neural crest cells: strategies to generate lineage diversifaction in vitro. 13: Avian cilliary and lumbar sympathetic ganglion neurons. 14: Enteric ganglion cells. 15: Mouse olfactory epithelium. 16: Schwann cell culture

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Neural cell culture : a practical approach

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