High performance liquid chromatography : fundamental principles and practice

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書誌事項

High performance liquid chromatography : fundamental principles and practice

edited by W.J. Lough and I.W. Wainer

Blackie Academic & Professional, 1995 , c1996

1st ed

  • : pbk

大学図書館所蔵 件 / 全3件

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

目次

INTRODUCTION, W.J. Lough and I.W. Wainer Analysis and Chromatography Historical Development of Chromatography HPLC Today EFFICIENCY, RETENTION, SELECTIVITY, AND RESOLUTION IN CHROMATOGRAPHY, C.M. Riley Introduction Chormatographic Mobility Peak Shape Retention Relationships Band Broadening and Column Efficiency Separation MODES OF CHROMATOGRAPHY, C.M. Riley Terminology Chromatographic Format Models of Retention Choice of Mode of Chromatography Normal-Phase Liquid Chromatography Reversed-Phased Liquid Chromatography Ion-Exchange Chromatography Size-Exclusion Chromatography SUPPORT MATERIALS AND SOLVENTS, P. Hambleton Adsorption Chromatography Chemically Bonded Silica Polymer Packings Porous Graphitic Carbon Solvents for HPLC INSTRUMENTATION: PUMPS, INJECTORS, AND COLUMN DESIGN, T. Noctor Introduction Types of HPLC Pump Approaches to the Reduction of Flow Pulsation The Modern HPLC Pump Valve Injectors Column Dimemsions Column Inlet/Outlet Connecting Tubing and Unions Conclusion INSTRUMENTATION: DETECTORS AND INTEGRATORS, D.K. Lloyd Ideal Detectors and Real Detectors Detector Performance Criteria The UV-Visible Absorbance Detector The Fluorescence Detector The Refractive Index Detector Electrochemical Detectors Other Detection Methods Integrators METHOD DEVELOPMENT AND QUANTITATION, W.J. Lough and I.W. Wainer Method Development Optimisation Relative Assays Method Validation Types of Quantitative Method SAMPLE PREPARATION, D. Stevenson Introduction Sample Preparation Procedures Matrix Properties Analyte Propeties Examples of Applications Automation Concluding Remarks POLYMER ANALYSIS, A.J. Handley Introduction Chromatography Modes Used for Polymer Analysis Size Exclusion Chromatography Other Modes of Chromatography HPLC IN BIOMEDICAL AND FORENSIC ANALYSIS, D. Perrett and P. White Introduction Biomedical Analysis Forensic Analysis ENVIRONMENTAL ANALYSIS, P.J. Renne Introduction HPLC Mode Sample Preparation Choice of Column Pumping System Detectors Sample Introduction Analytes Miscellaneous Suggested Protocol Conclusions FOOD, ORGANIC, AND PHARMACEUTICAL APPLICATIONS, W.J. Lough and I.W. Wainer Introduction Bulk Substances Products or 'Formulations' Conclusion

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