Epithelial cell culture : a practical approach

著者

    • Shaw, Andrew J.

書誌事項

Epithelial cell culture : a practical approach

edited by Andrew J. Shaw

(The practical approach series, 166)

IRL Press at Oxford University Press, c1996

  • : pbk
  • : hbk

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注記

Includes bibliographical references and index

内容説明・目次

巻冊次

: pbk ISBN 9780199635726

内容説明

This book describes methods of culturing epithelial cells so as to mimic accurately their behaviour in vivo, and the resulting model systems can be used in a wide range of research areas: pharmaceutics, pharmacology, toxocology, and cell biology. The culture technique involves growing cells on a porous substrate, so that the cells can receive nutrients by diffusion from the underlying layer, as they would in vivo.

目次

  • 1. Modelling epithelial tissues
  • 2. Identification and localization of cytokeratins
  • 3. Assessment of cell polarity
  • 4. Xenobiotic metabolism in epithelial cells
  • 5. Culture and characterization of human endothelial cells
  • 6. Studying transport processes in aborptive epithelia
  • 7. A model of glandular epithelium for studying secretion
  • 8. A model of the blood-testes barrier for studying testicular toxicity
  • 9. Reconstruction of human skin epidermis in vitro
  • Appendix 1. Addresses of suppliers
  • Appendix 2. An in vitro model of the blood-brain barrier for studying drug transport to the brain
巻冊次

: hbk ISBN 9780199635733

内容説明

This text describes methods of culturing epithelial cells so as to mimic accurately their behaviour in vivo. The resulting model systems can be used in a wide range of research areas: pharmaceutics, pharmacology, toxicology and cell biology. The culture technique involves growing cells on a porous substrate, so that the cells can receive nutrients by diffusion from the underlying layer, as they would in vivo.

目次

1: Modelling epithelial tissues. 2: Identification and localization of cytokeratins. 3: Assessment of cell polarity. 4: Xenobiotic metabolism in epithelial cells. 5: Culture and characterization of human endothelial cells. 6: Studying transport processes in aborptive epithelia. 7: A model of glandular epithelium for studying secretion. 8: A model of the blood-testes barrier for studying testicular toxicity. 9: Reconstruction of human skin epidermis in vitro. Appendix 1. Addresses of suppliers. Appendix 2. An in vitro model of the blood-brain barrier for studying drug transport to the brain

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