Epithelial cell culture : a practical approach
著者
書誌事項
Epithelial cell culture : a practical approach
(The practical approach series, 166)
IRL Press at Oxford University Press, c1996
- : pbk
- : hbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199635726
内容説明
This book describes methods of culturing epithelial cells so as to mimic accurately their behaviour in vivo, and the resulting model systems can be used in a wide range of research areas: pharmaceutics, pharmacology, toxocology, and cell biology. The culture technique involves growing cells on a porous substrate, so that the cells can receive nutrients by diffusion from the underlying layer, as they would in vivo.
目次
- 1. Modelling epithelial tissues
- 2. Identification and localization of cytokeratins
- 3. Assessment of cell polarity
- 4. Xenobiotic metabolism in epithelial cells
- 5. Culture and characterization of human endothelial cells
- 6. Studying transport processes in aborptive epithelia
- 7. A model of glandular epithelium for studying secretion
- 8. A model of the blood-testes barrier for studying testicular toxicity
- 9. Reconstruction of human skin epidermis in vitro
- Appendix 1. Addresses of suppliers
- Appendix 2. An in vitro model of the blood-brain barrier for studying drug transport to the brain
- 巻冊次
-
: hbk ISBN 9780199635733
内容説明
This text describes methods of culturing epithelial cells so as to mimic accurately their behaviour in vivo. The resulting model systems can be used in a wide range of research areas: pharmaceutics, pharmacology, toxicology and cell biology. The culture technique involves growing cells on a porous substrate, so that the cells can receive nutrients by diffusion from the underlying layer, as they would in vivo.
目次
1: Modelling epithelial tissues. 2: Identification and localization of cytokeratins. 3: Assessment of cell polarity. 4: Xenobiotic metabolism in epithelial cells. 5: Culture and characterization of human endothelial cells. 6: Studying transport processes in aborptive epithelia. 7: A model of glandular epithelium for studying secretion. 8: A model of the blood-testes barrier for studying testicular toxicity. 9: Reconstruction of human skin epidermis in vitro. Appendix 1. Addresses of suppliers. Appendix 2. An in vitro model of the blood-brain barrier for studying drug transport to the brain
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