Antibody engineering : a practical approach
著者
書誌事項
Antibody engineering : a practical approach
（The practical approach series, 169）
IRL Press, c1996
- : hbk
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199635924
内容説明
Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. This new Practical Approach book details protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities.
目次
- 1. Construction and use of antibody gene repertoires
- 2. Affinity maturation of antibodies using phage display
- 3. Human antibody repertoires in transgenic mice: manupulation and transfer of YACs
- 4. Measuring antibody affinities in solution
- 5. Measuring antibody affinities using Bio-sensors
- 6. Analysis of human antibody sequences
- 7. Rodent to human antibodies by CDR grafting
- 8. Rodent to human antibodies by phage display
- 9. Choosing and manipulating effector functions
- 10. Secretion and purification of antibody fragments expressed in bacteria
- 11. Production of single chain Fv monomers and multimers
- 12. Expression of immunoglobulin genes in mammalian cells
- 13. In vitro linkage of antibody fragments into dimeric and multimeric forms
- 巻冊次
-
: hbk ISBN 9780199635931
内容説明
Recombinant DNA techniques have revolutionized the isolation and production of antibodies. This volume describes methods and technologies which will allow the researcher to isolate a new antibody, analyse its properties, format the correct antibody or fragment, and produce sufficient quantities for experimental use. Topics in this volumes include: generation and analysis of antibodies; antibody repertoires; antibody screening and selection; measuring antibody affinities; sequence analysis; antibody engineering and production; conversion of rodent antibodies to human antibodies by CDR grafting or guided selection; choosing and optimizing effector functions; preparation and use of antibody-based molecules in eukaryotic and prokaryotic systems; scaling up manufacture; and generation of high-affinity high-specificity human antibodies in appropriate formats. Antibody Engineering is a unique manual of recombinant DNA methods for all those working with antibodies in research, diagnosis, and therapeutics.
Each chapter is written by a leading researcher in the field and provides essential background information, fully tested protocols, sample data from using these methods, trouble-shooting comments, and key hints and tips for success. This book is intended for post-graduates, post-doctoral researchers, and other academic staff involved in both clinical and molecular biological applications of antibodies.
目次
1: Construction and use of antibody gene repertoires. 2: Affinity maturation of antibodies using phage display. 3: Human antibody repertoires in transgenic mice: manupulation and transfer of YACs. 4: Measuring antibody affinities in solution. 5: Measuring antibody affinities using Bio-sensors. 6: Analysis of human antibody sequences. 7: Rodent to human antibodies by CDR grafting. 8: Rodent to human antibodies by phage display. 9: Choosing and manipulating effector functions. 10: Secretion and purification of antibody fragments expressed in bacteria. 11: Production of single chain Fv monomers and multimers. 12: Expression of immunoglobulin genes in mammalian cells. 13: In vitro linkage of antibody fragments into dimeric and multimeric forms
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