Biofilms : methods for enzymatic release of microorganisms

著者

書誌事項

Biofilms : methods for enzymatic release of microorganisms

Jean F. Brisou

CRC Press, c1995

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注記

Includes bibliographical references (p. 181-192) and index

内容説明・目次

内容説明

This text presents a new technique for detecting microorganisms, specifically bacteria found in all levels of the biosphere. It also discusses methods for enzymatic release of these microbes as well as their interactions in all ecosystems. Drawings and micrographs help to illustrate this concept. Part one is devoted to the mechanisms of adherence. The second part discusses microbial ecology and the bacterial population of tissues as well as both land and aquatic microbiocenoses in general. Part three specifically covers technique. What is known about the mechanisms of adherence justifies the choice of techniques suggested. Applications in areas such as nature, medicine, environmental hygiene and the food industry are discussed. The explanation of useful techniques, the author's research results, and practical application methods make this volume an essential reference tool for researchers, technicians and practitioners.

目次

Part I: Adherence in Microbiology Structures and Mechanisms Definition Review of Membrane Structures Membrane Proteins Renewal of Membrane Constituents Adherence Mechanisms Adsorption-Ionic Zone The Roles of Polycations Adhesion-Second Reversible Stage Irreversible Adherence-Adhesins Glycocalyx Fibrillae Other Possible Adhesins Spores A Few Genetic Reminders Chemical Elements of Adherence The Bacterial Wall Polysaccharides Biosynthesis of Polysaccharides Main Bacterial Polysaccharides Lipopolysaccharides Comment Proteins Polypeptides and Derivates Lipoproteins and Derivative Complexes Particularities of Mycobacteria Composition Differences of the Bacterial Walls Part II: Bacteria Life in the Wild Adherence in Living Tissues Cellular Receptors Adherence to a Few Organs and Organisms Man and Animals Microbisms of the Mouth and Teeth Microbisms of the Digestive Tube Microbisms of the Stomach Microbisms of the Urogenital Apparatus Natural Microbiocenoses Vegetals The Rhizosphere Soils and Sediments Aquatic Environments Gas/Liquid Interfaces Nonmiscible Liquid Interfaces Microbial Activity on Interfaces Role of Plankton in Aquatic Environments Polymorphism of Bacteria in Nature, Agglomerates Inert Surfaces Part III: Enzymatic Release Techniques of Bacteria Basic Techniques Introduction Attack of Host Sites or Substrates Inorganic or Inert Surfaces Organic Surfaces Analysis of North Atlantic Seawater Useable Enzymes Optimal Conditions of Useable Enzymes Practical Applications Techniques and Methods Quantitation of the Enzymes Conservation of the Enzyme Solutions Standard Method Specific Method Stages of Release Experiments With Only Enzymes On Marine Sediment Experimentation on Mussel Grindings Treatment of Infected Urine Experiment on Inert Powders Association of Enzymes-Antibiotics Experiment Using Bacteria Marked With Radioelements Special Case of Fat Experiment by Richelle Maurer Experiment by E. Ebiou Experiment by Patrick Brisou Postface Bibliography Index

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