New methods in peptide mapping for the characterization of proteins

書誌事項

New methods in peptide mapping for the characterization of proteins

edited by William S. Hancock

(CRC series in analytical biotechnology)

CRC Press, c1996

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.

目次

Peptide Mapping to Characterize Subtle Modifications in the Analysis of Protein Pharmaceuticals, V.T. Ling, M.L. Eng, P.-J. Lee, R.G. Keck, B.A. Keyt, and E.Canova-Davis The Optimization of Peptide Mapping via Computer Simulation, L.R. Snyder The Use of Capillary Electrophoresis for Peptide Mapping of Proteins, E. Rickard and J. Towns Packed Capillary HPLC-ELectrospray Ionization Mass Spectrometry, J. Stults, B.L. Gillece-Castro, W.J. Henzel, J.H. Bourell, K.L. O'Connell, and L.M. Nuwaysir Capillary Electrophoresis/Mass Spectrometry in Peptide Mapping, J. Wahl, H. Udseth, and R. Smith Analyzing Reversed Phase Peptide Maps of Recombinant Glycoproteins, A. Guzzetta, W. Hancock Matrix-Assisted Laser Desorption Mass Spectrometry for Peptide Mapping, P. Lecchi and R. Caprioli

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詳細情報

  • NII書誌ID(NCID)
    BA28606900
  • ISBN
    • 0849378222
  • LCCN
    95034181
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    Boca Raton, FL
  • ページ数/冊数
    246 p.
  • 大きさ
    25 cm
  • 分類
  • 件名
  • 親書誌ID
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