In situ hybridization techniques for the brain
著者
書誌事項
In situ hybridization techniques for the brain
(IBRO handbook series : methods in the neurosciences, v. 17)
Wiley & Sons, 1996
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
"In situ" Hybridization is a powerful histochemical technique that is widely used. This book provides a comprehensive listing of protocols and includes both the theoretical background to "in situ" Hybridization and specific technical details, enabling the reader to plan and carry out the procedures in their own laboratories. This book is targeted towards newcomers to the techniques and takes a back-to-basics approach. It includes details on both routine procedures and developments, and the interpretation of data.
目次
- A survey of the "in situ" Hybridization technique
- molecular biological techniques for the "in situ" Hybridization involving the use of bacterial cells
- "in situ" Hybridization with [S35] - labelled oligonucleotides
- "in situ" Hybridization with [S35] - and digoxigenin-labelled RNA probes
- combined immunocytochemistry and "in situ" Hybridization
- the use of human post-mortem brain tissue for "in situ" Hybridization - practical considerations
- production of labelled crna probes useful for "in situ" Hybridization histochemistry without vector cloning
- quantitative analysis and interpretation of data for "in situ" Hybridization at the cellular level.
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