Clinical gene analysis and manipulation : tools, techniques, and troubleshooting

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書誌事項

Clinical gene analysis and manipulation : tools, techniques, and troubleshooting

by Janusz A.Z. Jankowski, editor ; Julia M. Polak, deputy editor ; foreword, Sir David Weatherall

（Postgraduate medical science）

Published in association with the Royal Postgraduate Medical School, University of London by Cambridge Unversity Press, 1996

  • hardcover
  • pbk.

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This practical compendium provides clinical scientists with an essential guide to the basic techniques of molecular medicine. It serves as a laboratory manual and a source of reference. It is suitable for those wishing to perform basic semi-quantitative experiments such as Northern or Southern blots and also those wishing to undertake more specialised genetic manipulations such as gene cloning, expression and creation of DNA libraries. It will give clinical scientists a unique insight into the potential of these techniques. As stated by Sir David Weatherall: 'It should be of great value to both established research workers and young scientists coming into the field for the first time. It deserves every success.'

目次

  • Foreword Sir D. Weatherall
  • Part I. Introduction, Background and Essential Theory: 1. Introduction and applications J. Jankowski
  • 2. History of molecular genetics R. Chinery
  • 3. Molecules of gene expression (protein, RNA and DNA) R. Chinery
  • Part II. Molecular Cell Tools: 4. Tissue analysis and morphological characterisation
  • 5. Cellular analysis and cell culture
  • 6. Chromosomal and molecular analysis
  • 7. Gene manipulation F. Bedford and J. Jankowski
  • Part III. Molecular Cell Techniques: 8. Cellular analysis
  • Initiation and propagation of cultured cell lines I. Freshney
  • Characterisation of cultured cell cines S. Kirkland and K. Henderson
  • Cytoxicity assays in vitro R. Phillips and B. Boullier
  • The production of monoclonal and polyclonal antibodies using synthetic peptides as immunogens T. RajKumar and B. Gullick
  • 9. Tissue analysis
  • Modern morphological techniques in molecular cell analysis (Immunocytochemistry autoradiography in situ hybridisation) L. Gordon and A. Bishop
  • An overview of methodologies for assessing proliferation and cell death R. Dover
  • 10. Chromosomal and molecular assays
  • 11. Assessing proteins and nucleic acids quantitatively and qualitatively by gel fractionation and related techniques
  • 12. Extraction of protein, western blotting, special protein assays - RIA and ELISA
  • 13.Extraction of RNA and northern blot and RT PCR
  • 14.Extraction of mammalian DNA, PCR, Southern blot and dot blot
  • 15. Production of primers/probes J. Jankowski and F. Bedford
  • 16. Molecular cytogenetic analysis of tumours and cell lines and its application in biology and medicine
  • 17. Cytogenetics, FISH, allelle loss S. Jhanwar
  • Analysis of DNA sequence polymorphisms and screening for point mutations A. Wright and J. Prosser
  • 18. D. Gene manipulation
  • 19. Molecular level
  • 20. Basic cloning techniques
  • 21. Purification of bacterial, plasmid or phage DNA cloning in prokaryotes and construction of recombinant DNA molecules S. Legon
  • Creation and screening of cDNA libraries
  • 22. A practical approach to cloning J. Goldenring and I. Modlin
  • 23. Identification and isolation of novel DNA and cDna species F. Bedford and J. Jankowski
  • 24. Manual and automated DNA and RNA sequencing M. Jones
  • 25. Assessment of gene expression N. Bates and H. Hurst
  • 26. Cellular level
  • 27. Delivery of DNA into cells
  • 28. Production of competent cells and cell transfection S. Castledon and R. Vile
  • 29. Antisense RNA technology (Oligodeoxyribonucleotides, ribozymes and retroviruses) P. L. Iverson and I. Baysever
  • 30. IV. Trouble-shooting: (i) Microbial contamination during cell culture K. Henderson
  • 31.(ii) Safety aspects of genetic manipulation work S. Legon
  • 32. (iii) tissue storage and preparation J. Jankowski
  • 33. (iv) Failed experiments - general guidance J. Jankowski
  • 34. (v) Selection of adequate controls J. Jankowski
  • 35. (vi) Quality control J. Jankowski
  • 36. V. Gene manipulation and applications to molecular medicine: 37. Inherited genetic disease and developmental disorders P. Buss
  • 38. Oncology of solid tumours J. Jankowski
  • 39. Oncology of haematogenous cancers S. Ridge
  • 40. genetic therapies K. Sikora
  • VI. Appendices: A. Selected bibliography
  • B. Commercial suppliers
  • 41. Equipment (quantitative gel analysis and gene manipulation)
  • 42. Consumables
  • 43. Solutions.

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  • Postgraduate medical science

    Published in association with the Royal Postgraduate Medical School, University of London by Cambridge University Press

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