Protein structure : a practical approach
著者
書誌事項
Protein structure : a practical approach
（The practical approach series, 174）
IRL Press, c1997
2nd ed
- : hbk
- : pbk
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注記
Includes bibliographies and index
内容説明・目次
内容説明
This work provides general methods for determining protein structure, from the simple electrophoretic, chromatographic, and spectrophotometric techniques to the relatively more complex and sophisticated methods of mass spectrometry and ultracentrifugation. This second edition has been updated to embrace the developments in the field, and should be of value to all involved in protein research. It is intended for graduates and post-doctoral researchers, academic staff, biotechnology professionals in the field of protein science; protein structure is also of relevance to biochemists.
目次
- Protein molecular weight determination by sodium dodecyl sulfate polyacrylamide gel electrophoresis
- protein analysis by mass spectrometry
- immunological detection of proteins of known sequence
- identification of common post-translational modifications
- peptide mapping
- counting integral numbers of residues by chemical modification
- disulfide bonds between cysteine residues
- analysis of protein conformation by gel electrophoresis
- hydrodynamic properties of proteins
- how to determine the molar absorbance coefficient of a protein
- optical spectroscopy to characterize protein confirmation and conformational changes
- measuring the conformational stability of a protein
- immunochemical analysis of protein conformation
- stabilization of protein structure by solvents.
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