Immobilization of enzymes and cells

書誌事項

Immobilization of enzymes and cells

edited by Gordon F. Bickerstaff

(Methods in biotechnology, 1)

Humana Press, c1997

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This collection of today's most significant immobilization methods reproduces those procedures currently and widely used in both industrial and research laboratories. These unfailingly reproducible techniques are so crafted that users can easily incorporate them into their existing methods and processes. Extensive notes provide additional information that helps extend the flexibility of the method.

目次

Immobilization of Enzymes and Cells: Some Practical Considerations, Gordon F. Bickerstaff. Immobilization of Enzymes by Selective Adsorption on Biotinylaminopropyl Celite or Glass, Harold E. Swaisgood, Xiaolin L. Huang, and Marie K. Walsh. Immobilization of Proteins on Thionyl Chloride-Activated Controlled-Pore Glass, Violeta G. Janolino and Harold E. Swaisgood. Enzyme Immobilization on Nylon, Pedro Lozano and Jose L. Iborra. Visual Assessment of Enzyme Immobilization, Arturo Manjon and Jose L. Iborra. Immobilization in Carrageenans, Jose L. Iborra, Arturo Manjon, and Manuel Canovas. Entrapment in Calcium Alginate, Jane E. Fraser and Gordon F. Bickerstaff. Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports, Maria Cantarella, Francesco Alfani, Laura Cantarella, and Alberto Gallifuoco. Activation of Rayon/Polyester Cloth for Protein Immobilization, Hiroshi Yamazaki and C. Scott Boyd. Immobilization of Enzymes on Microelectrodes Using Chemical Crosslinking, Milena Koudelka-Hep, Nico F. de Rooij, and David J. Strike. Photolithographic Patterning of Enzyme Membranes for the Modification of Microelectrodes, Milena Koudelka-Hep, Nico F. de Rooij, and David J. Strike. Electrochemical-Based Immobilization of Enzymes, David J. Strike, Nico F. de Rooij, and Milena Koudelka-Hep. Immobilization of Enzymes on Thermo-Responsive Polymers, Kazuhiro Hoshino, Setuko Akakabe, Shoichi Morohashi, and Toshisuke Sasakura. Immobilization of Photosynthetic Membranes in an Albumin-Glutaraldehyde Crosslinked Matrix, Robert Carpentier. Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization, Guy Fortier, Nicole Demers, Jacques-Jean Francois, Jean-Charles Gayet, and Edith M. D'Urso. Poly(Carbamoyl Sulfonate) Hydrogels, Andreas Muscat and Klaus-Dieter Vorlop. Enzyme Immobilization on Polyethyleneimine-Coated Magnetite Particles, Gilbert Bardeletti. Immobilization of Enzymes and Proteins on Red Blood Cells, Laura Chiarantini and Mauro Magnani. Cellulose Paper Support for Immobilization, Marion Paterson and John F. Kennedy. Immobilization of Cells Using Electrostatic Droplet Generation, Mattheus F. A. Goosen, Eltag S. E. Mahmud, Abdullah S. Al-Ghafri, Hamad A. Al-Hajri, Yousuf S. Al-Sinani, and Branko Bugarski. Hepatocyte Immobilization in Agarose and Functional Integrity Testing, Hassan Farghali and Sixtus Hynie. Immobilized Hepatocytes in Xenobiotic Biotransformation Studies, Sixtus Hynie, Ludmila Kamenikova, and Hassan Farghali. Immobilization of Liposomes and Proteoliposomes in Gel Beads, Eggert Brekkan, Qing Yang, Gerhard Viel, and Per Lundahl. Cell Immobilization with Phosphorylated Polyvinyl Alcohol (PVA) Gel, Kuo-Cheng Chen and Jer-Yiing Houng. Covalent Immobilization of Enzymes to Graphitic Particles, Marco F. Cardosi. Enzyme Immobilization Using Chitosan-Xanthan Complexes, Severian Dumitriu, Pierre Vidal, and Esteban Chornet. Calcium Alginate Film Formed on a Stainless Steel Mesh, Harold E. Swaisgood and Flavia M. L. Passos. Preparation of Immobilized Subunits of a Multisubunit Enzyme, Gordon F. Bickerstaff. Characterization of Enzyme Activity, Protein Content, and Thiol Groups in Immobilized Enzymes, Gordon F. Bickerstaff. Immobilization of Enzymes Acting on Macromolecular Substrates: Reduction of Steric Problems, Jose M. Guisan, Guadalupe Penzol, Pilar Armisen, Agatha Bastida, Rosa M. Blanco, Roberto Fernandez-Lafuente, and Eduardo Garcia-Junceda. Immobilization of Enzymes on Glyoxyl-Agarose: Strategies for Enzyme Stabilization by Multipoint Attachment, Jose M. Guisan, Agatha Bastida, Rosa M. Blanco, Roberto Fernandez-Lafuente, and Eduardo Garcia-Junceda. Stabilization of Immobilized Enzymes by Chemical Modification with Polyfunctional Macromolecules, Jose M. Guisan, Veronica Rodriguez, Cristina M. Rosell, Gloria Soler, Agatha Bastida, Rosa M. Blanco, Roberto Fernandez-Lafuente, and Eduardo Garcia-Junceda. Covalent Immobilization of Enzymes Using Commercially Available CDI-Activated Agarose, George J. Piazza and Marjorie B. Medina. Immobilization of Cells in Polyelectrolyte Complexes, Johanna Mansfeld and Horst Dautzenberg. Coimmobilization of Enzymes and Cells, Johanna Mansfeld and Horst Dautzenberg. Adsorption of Lipase on Inorganic Supports, Jose V. Sinisterra. Immobilization of Enzymes on Inorganic Supports by Covalent Methods, Jose V. Sinisterra. Use of Divalent Metal Ions Chelated to Agarose Derivatives for Reversible Immobilization of Proteins, Robert R. Beitle, Jr. and Mohammad M. Ataai. Transition Metal Methods for Immobilization of Enzymes and Cells, John F. Kennedy, Joaquim M. Cabral, Maria R. Kosseva, and Marion Paterson. Index.

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