Subcellular fractionation : a practical approach
Author(s)
Bibliographic Information
Subcellular fractionation : a practical approach
（The practical approach series, 173）
IRL Press at Oxford University Press, c1997
- : hbk
- : pbk
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers many necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.
Table of Contents
1.: Homogenization of tissues and cells. 2.: Isolation of subcellular fragments. 3.: Isolation and characterization of nuclei and nuclear subfractions. 4.: Subcellular fractionation of mitochondria. 5.: Isolation and characterization of peroxisomes. 6.: Lysosomes and endocytosis. 7.: The membranes of the secretory and exocytic pathways. 8.: Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells. 9.: Ribosomes and polysomes. 10.: Electron microscopy of organelles
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