Epitope mapping protocols
Author(s)
Bibliographic Information
Epitope mapping protocols
(Methods in molecular biology / John M. Walker, series editor, 66)
Humana Press, c1996
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
Interest in epitope mapping, or finding out where antibodies bind to their antigens, is by no means restricted to immunologists, but is shared by biolo gists from a wide range of disciplines in which antibodies are used as molecu lar reagents. The epitope mapper may be interested in studying protein-protein interactions, in developing an immunoassay, in producing protective peptide vaccines, in investigating the pathogenesis of autoimmune diseases, or in defining protein topology in intact cells or organelles, to mention only a few of the possibilities. The aim of Epitope Mapping Protocols is to provide both a useful range of alternative practical methods for the experienced mapper and a fairly com prehensive introduction for someone embarking on antibody production and mapping for the first time. Contributors were encouraged to illustrate their protocols with results from their own research and most of them elected to do so. After an introductory chapter, the protocols are arranged in three groups: The first group of twelve methods uses (or can use) whole, native antigens and may therefore be suitable for conformational epitopes; the second group of five uses peptides or peptide libraries; and the third group of eleven requires antigen expressed from recombinant DNA. Within this last group, methods more likely applicable to conformational epitopes are placed first.
Table of Contents
Overview: Choosing a Method for Epitope Mapping
Glenn E. Morris
Crystallographic Studies of Antigen-Antibody Interactions
Frederick A. Saul and Pedro M. Alzari
Epitope Mapping Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy
Irina Kustanovich and Anat Zvi
Mapping Epitopes on Antigens by Immunodiffusion in Gel
Giuseppe A. Molinaro and William C. Eby
A Simple Solid-Phase Competition Assay with Labeled Antigen
Masahide Kuroki
Epitope Mapping by Antibody Competition: Methodology and Evaluation of the Validity of the Technique
Socrates J. Tzartos
Epitope Mapping by Surface Plasmon Resonance in the BIAcore
Berit Johne
Identifying Residues in Antigenic Determinants by Chemical Modification
N. Martin Young and Raymond P. Oomen
Epitope Mapping by Differential Chemical Modification of Antigens
Hans Rudolf Bosshard
Epitope Mapping by Proteolysis of Antigen-Antibody Complexes: Protein Footprinting
Ronald Jemmerson
Proteolytic Fragmentation for Epitope Mapping
Maria R. Mazzoni, Nickolai O. Artemyev, and Heidi E. Hamm
Epitope Mapping by Chemical Fragmentation
Glenn E. Morris
Probing Antibody-Antigen Interactions by Mass Spectrometry
Yingming Zhao and Brian T. Chait
Epitope Mapping Using Multipin Peptide Synthesis
Stuart J. Rodda, N. Joe Maeji, and Gordon Tribbick
SPOT Synthesis: Epitope Analysis with Arrays of Synthetic Peptides Prepared on Cellulose Membranes
Ronald Frank and Heike Overwin
Tea Bag Synthesis of Positional Scanning Synthetic Combinatorial Libraries and Their Use for Mapping Antigenic Determinants
Clemencia Pinilla, Jon R. Appel, and Richard A. Houghten
Epitope Mapping Using Phage-Displayed Peptide Libraries
Diane Dottavio
Reiterative Screening of Phage-Display Peptide Libraries, with Antibodies
Alexander Pereboev and Glenn E. Morris
Homolog Scanning
Lin-Fa Wang
Epitope Mapping Using an Oligonucleotide Replacement Method
Hannah Alexander
Epitope Mapping by Region-Specified PCR Mutagenesis
Takehiko Shibata and Masayuki Ikeda
Random Fragment Libraries Using Yeast Expression Plasmid
Serge Benichou and Genevieve Inchauspe
Epitope Mapping Using Random Fragment Expression Libraries in l Phages
Hartmut Porzig and Kenneth D. Philipson
Random Fragment Libraries Displayed on Filamentous Phage
Lin-Fa Wang and Meng Yu
Epitope Mapping by Expression of Restriction Enzyme or PCR Fragments in Bacterial Plasmids
Johannes A. Lenstra and Arnoud H. M. Van Vliet
Epitope Mapping of Protein Antigens by Expression-PCR (E-PCR)
David E. Lanar, Kevin C. Kain, and Henry B. Burch
Epitope Mapping on Extracellular Domains of Cell-Surface Proteins Using Exonuclease III
Thomas Brummendorf, Antonius Plagge, and Ullrich Treubert
An Improved Method for Mapping Epitopes of Recombinant Antigens by Transposon Mutagenesis
Steven G. Sedgwick, Brian A. Morgan, Nguyen thi Man, and Glenn E. Morris
Incomplete Polypeptides of In Vitro Translation for Epitope Localization
Lisa Djavadi-Ohaniance and Bertrand Friguet
T-Cell Epitope Mapping with Synthetic Peptides and Peripheral Blood Mononuclear Cells
Stuart J. Rodda
Use of Natural or Selected Mutants and Variants for Epitope Mapping
Glenn E. Morris
Production of Panels of Monoclonal Antibodies by the Hybridoma Method
Nguyen thi Man and Glenn E. Morris
Production of Phage-Display Antibodies for Epitope Mapping
Jenny Walker and George Banting
Index
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