Protein function : a practical approach
著者
書誌事項
Protein function : a practical approach
（The practical approach series, 175）
IRL Press at Oxford University Press, c1997
2nd ed
- : hbk
- : pbk
- : set : hbk
- : set : pbk
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注記
Companion vol. to: Protein structure. 2nd ed. 1997
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199636150
内容説明
The companion to this book, Protein Structure: A Practical Approach, gives methods of determining the structure of a newly discovered protein, but that is only part of the story. Protein Function: A Practical Approach completes the picture by providing the techniques for elucidating the functional properties of proteins, which can vary enormously, from simple structural roles to catalysis, from information transmission to energy control. This
collection of tried-and-tested protocols will be applicable to many proteins, and can be performed in an average laboratory, without the need for specialized equipment.
目次
- 1. Identifying proteins by two-dimensional gel
- electrophoresis
- 2. Expressing cloned genes in Escherichia coli
- 3. Folding proteins
- 4. Ligand binding
- 5. Ligand blotting
- 6. Affinity labelling
- 7. Chemical cross-linking and protein function
- 8. Analysis of sequence-specific DNA-binding proteins
- 9. Purification of sequence-specific DNA-binding proteins by affinity chromatography
- 10. Chemical modification
- 巻冊次
-
: hbk ISBN 9780199636167
内容説明
The companion to this book, "Protein structure: a practical approach", shows methods of determining the structure of a newly discovered protein, but that is only part of the story. This text completes the picture by providing the techniques for elucidating the functional properties of proteins, which can vary enormously, from simple structural roles to catalysis, from information transmission to energy control. This collection of tried-and-tested protocols will be applicable to many proteins, and can be performed in an average laboratory, without the need for specialized equipment.
目次
- Identifying proteins by two-dimensional gel
- electrophoresis
- expressing cloned genes in escherichia coli
- folding proteins
- ligand binding
- ligand blotting
- affinity labelling
- chemical cross-linking and protein function
- analysis of sequence-specific DNA-binding proteins
- purification of sequence-specific DNA-binding proteins by affinity chromatography
- chemical modification.
- 巻冊次
-
: set : hbk ISBN 9780199636174
内容説明
The first editions of Protein Structure and Protein Function were enormously popular; they have now been fully revised and brought up to date. These two-volume sets - hardback and paperback - offer a considerable saving on the price of the individual volumes.
- 巻冊次
-
: set : pbk ISBN 9780199636204
内容説明
The first editions of Protein Structure and Protein Function were enormously popular; they have now been fully revised and brought up to date. These two-volume sets - hardback and paperback - offer a considerable saving on the price of the individual volumes.
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