Antibody applications : essential techniques
Author(s)
Bibliographic Information
Antibody applications : essential techniques
（Essential techniques series）
Wiley , Published in association with BIOS Scientific Publishers, c1995
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This convenient lab manual gives quick and easy reference to key protocols and practical tips needed to undertake antibody applications. Thirty-five protocols are listed including a troubleshooting guide and tips to indicate hazards, good points to stop methods, key conditions, and reagent disposal.
Table of Contents
- INTRODUCTORY MATERIAL: Characterization of Antibodies
- Labelling of Antibodies
- Applications of Antibodies
- PROTOCOLS: Antibody Concentration by Optical Density
- Assessment of Purity of Antibody Preparation using SDS-PAGE
- Measurement of Affinity using Friguet Method
- Labelling of Antibodies with Biotin
- Labelling of Antibodies with 125I
- Labelling of Antibodies with Fluorochrome
- Labelling of Antibodies with Enzyme
- Direct ELISA
- Sandwich ELISA
- Competitive ELISA for Antigen Detection
- Competitive ELISA for Epitope Mapping
- ELISPOT
- Fluid Phase Radioimmunoassay
- Cell Surface Immunofluorescence
- Intracellular Immunofluorescence on Cytospines
- Intracellular Immunofluorescence on Cell Suspensions
- Immunofluorescence on Tissue Sections
- Enzyme-Linked Immunohistology
- Immunoaffinity Chromatography using Columns
- Batch Immunoaffinity Chromatography
- Immunoprecipitation of Fluid Phase Antigen
- Immunoprecipitation of Cellular Antigens
- Ouchterlony Double Immunodiffusion
- Single Radial Immunodiffusion.
by "Nielsen BookData"