Antibody applications : essential techniques
著者
書誌事項
Antibody applications : essential techniques
（Essential techniques series）
Wiley , Published in association with BIOS Scientific Publishers, c1995
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This convenient lab manual gives quick and easy reference to key protocols and practical tips needed to undertake antibody applications. Thirty-five protocols are listed including a troubleshooting guide and tips to indicate hazards, good points to stop methods, key conditions, and reagent disposal.
目次
- INTRODUCTORY MATERIAL: Characterization of Antibodies
- Labelling of Antibodies
- Applications of Antibodies
- PROTOCOLS: Antibody Concentration by Optical Density
- Assessment of Purity of Antibody Preparation using SDS-PAGE
- Measurement of Affinity using Friguet Method
- Labelling of Antibodies with Biotin
- Labelling of Antibodies with 125I
- Labelling of Antibodies with Fluorochrome
- Labelling of Antibodies with Enzyme
- Direct ELISA
- Sandwich ELISA
- Competitive ELISA for Antigen Detection
- Competitive ELISA for Epitope Mapping
- ELISPOT
- Fluid Phase Radioimmunoassay
- Cell Surface Immunofluorescence
- Intracellular Immunofluorescence on Cytospines
- Intracellular Immunofluorescence on Cell Suspensions
- Immunofluorescence on Tissue Sections
- Enzyme-Linked Immunohistology
- Immunoaffinity Chromatography using Columns
- Batch Immunoaffinity Chromatography
- Immunoprecipitation of Fluid Phase Antigen
- Immunoprecipitation of Cellular Antigens
- Ouchterlony Double Immunodiffusion
- Single Radial Immunodiffusion.
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