Apoptosis techniques and protocols
著者
書誌事項
Apoptosis techniques and protocols
（Neuromethods, 29）
Humana Press, c1997
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
An indispensable compendium of state-of-the-art theory and detailed how-to instruction essential for understanding and studying apoptosis. The expert contributors cover such important topics as the histological, biological, and molecular criteria for apoptosis and programmed cell death; necrosis and apoptosis in the CNS; and cellular, invertebrate, animal, and human models of apoptosis in Alzheimer's disease, AIDS, and stroke. The techniques they describe examine the critical steps involved in the apoptosis process, and include PCR analysis of cell-cycle-regulated proteins, histochemical analysis of DNA regulation, DNA laddering analysis, and cytochemical alterations of living cells.
目次
Gene Tools to Study Neuronal Apoptosis Isabelle Martinou Harald Frankowski Marc Missotten Remy Sadoul and Jean-Claude Martinou. Detection of Apoptotic or Necrotic Death in Neuronal Cells by Morphological Biochemical and Molecular Analysis Martin Gschwind and Gerda Huber. Detection of Cell Death in Neural Tissue and Cell Culture Thomas Mahalik Malcolm Wood and Gregory Owens. Cultured Cerebellar Granule Neurons as a Model of Neuronal Apoptosis Guang-Mei Yan and Steven M. Paul. Optimization and Validation of RT-PCR as a Tool to Analyze Gene Expression During Apoptosis Steven Estus. p53 Induction Is a Marker of Neuronal Apoptosis in the Central Nervous System Shahin Sakhi and Steven S. Schreiber. Apoptosis and Ischemia in the Central Nervous System Matthew D. Linnik. Analyses of Apoptosis-Associated DNA Fragmentation In Vivo During Neurodegeneration of the Peripheral Olfactory System in Adult Mammals Emmanuel Moyse and Denis Michel. Both Irreversible Neuronal Death and Reversible Neuronal Stress Are Associated with Increased Levels of Statin a Marker of Cell Cycle Arrest Patricia Boksa Uwe Beffert Doris Dea Richard Alonso Dayan O'Donnell and Judes Poirier. b-Amyloid-Induced Neuronal Cell Death in Transgenic Mice and Alzheimer's Disease Frank M. LaFerla and Gilbert Jay. Use of TUNEL to Measure Retinal Ganglion Cell Apoptosis Lisa Ayn Kerrigan and Donald J. Zack. Methods for Detection of Apoptosis in the CNS Kathrin D. Geiger Floyd E. Bloom and Nora E. Sarvetnick. Techniques for Distinguishing Apoptosis from Necrosis in Cerebrocortical and Cerebellar Neurons Emanuela Bonfoco Maria Ankarcrona Dimitri Krainc Pierluigi Nicotera and Stuart A. Lipton. The Nematode Caenorhabditis elegans as a Model System to Study Neuronal Cell Death Paul Desjardins and St'phane Ledoux. Index.
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