Complement : a practical approach
Author(s)
Bibliographic Information
Complement : a practical approach
（The practical approach series, 182）
IRL Press, 1997
- : hbk.
- : pbk.
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Description and Table of Contents
- Volume
-
: pbk. ISBN 9780199635399
Description
The complement system is a collection of plasma enzymes that interact in a complex way as a reaction to cell injury, and is central to the immune response. The enzymes that make up the system and their interactions are very widely studied. This volume provides detailed practical protocols for quantitation of the complement components, inhibitors, and receptors, and methods for analysis of complement biosynthesis and for the study of complement in non-human
species.
Table of Contents
- 1. The complement system: an introduction
- 2. Haemolytic assays for whole complement activity and individual components
- 3. Immunochemical methods for the detection of complement components and complement activation
- 4. Identification, functional assessment, and quantitation of receptors and membrane regulatory proteins whose ligands are C3 and C4
- 5. C1 inhibitor and HANe
- 6. Formation and regulation of the membrane attack complex
- 7. Purification of complement components
- 8. The anaphylatoxins
- 9. Complement genetics
- 10. Biosynthesis of complement components
- 11. Complement and immune complexes
- 12. Non-human complement
- Volume
-
: hbk. ISBN 9780199635405
Description
The complement system is a collection of plasma enzymes that interact in a complex way as a reaction to cell injury, and is central to the immune response. The enzymes that make up the system and their interactions are very widely studied. This text contains detailed step-by-step protocols for analysis of the complement system, and is aimed at new and experienced workers in complement research, as well as clinicians with an interest in immunological diseases. The areas covered include functional and immunochemical methods for the quantitation of complement components, inhibitors, receptors, inflammatory effects of anaphylatoxin release, methods for the analysis of complement biosynthesis and for the study of the system in non-humans. Of particular interest to clinicians are chapters on complement and immune complexes, C2 Inh deficiency, and the genetic analysis of this highly polymorphic system. This book is intended for post-graduate and post-doctoral researchers, academic staff, and clinicians in the fields of biochemical immunology and immunological disease.
Table of Contents
1: The complement system: an introduction. 2: Haemolytic assays for whole complement activity and individual components. 3: Immunochemical methods for the detection of complement components and complement activation. 4: Identification, functional assessment, and quantitation of receptors and membrane regulatory proteins whose ligands are C3 and C4. 5: C1 inhibitor and HANe. 6: Formation and regulation of the membrane attack complex. 7: Purification of complement components. 8: The anaphylatoxins. 9: Complement genetics. 10: Biosynthesis of complement components. 11: Complement and immune complexes. 12: Non-human complement
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