Signalling by inositides : a practical approach
著者
書誌事項
Signalling by inositides : a practical approach
（The practical approach series）
IRL, 1997
- :hbk
- :pbk
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注記
Includes bibliographical references
内容説明・目次
- 巻冊次
-
:pbk ISBN 9780199636389
内容説明
The metabolism and functions of inositol phosphates impinge on various branches of biochemistry, physiology, and molecular biology, and methodological information is in consequence scattered far and wide. This book unites a selection of the most fundamental and commonly used techniques from leading international signal transduction laboratories, and brings together many valuable protocols for purifying and assaying inositides and related compounds. A novel feature is
a catalogue of non-commercial sources of synthetic inositide analogues.
目次
- 1. Assaying inositol phospholipid turnover in plant cells
- 2. Measurement of inositol phosphate turnover in intact cells and cell-free systems
- 3. Mass assay of inositol and its use to assay inositol polyphosphates
- 4. Phospholipase C-gamma 1
- 5. Baculovirus-promoted expression, purification, and functional assay of G-protein regulated PLC-beta isoenzymes
- 6. Purification and assay of phospholipase C-delta
- 7. The purification and assay of inositol polyphosphate-binding proteins
- 8. Mass assay of inositol 1,4,5-trisphosphate and phosphatidylinositol 4,5-bisphosphate
- 9. Desalting inositol polyphosphates by dialysis
- 10. The Ins(1,4,5)P3 receptor
- 11. Purification and assay of inositol hexakisphosphate kinase and diphosphoinositol pentakisphosphate kinase
- 12. Phosphoinositide 3-kinase
- 巻冊次
-
:hbk ISBN 9780199636396
内容説明
The metabolism and functions of inositol phosphates impinge on various branches of biochemistry, physiology, and molecular biology, and methodological information is in consequence scattered far and wide. This study unites a selection of the most fundamental and commonly used techniques from leading international signal transduction laboratories, and brings together many protocols for purifying and assaying inositides and related compounds. It also features a catalogue of non-commercial sources of synthetic inositide analogues. This study is designed for post-graduate and post-doctoral researchers and academic staff in biochemistry, physiology, and molecular biology.
目次
1: Assaying inositol phospholipid turnover in plant cells. 2: . Measureent of inositol phosphate turnover in intact cells and cell-free systems. 3: Mass assay of inositol and its use to assay inositol polyphosphates. 4: Phospholipase C-gamma 1. 5: Baculovirus-promoted expression, purification, and functional assay of G-protein regulated PLC-beta isoenzymes. 6: Purification and assay of phospholipase C-delta. 7: The purification and assay of inositol polyphosphate-binding proteins. 8: Mass assay of inositol 1,4,5-trisphosphate and phosphatidylinositol 4,5-bisphosphate. 9: Desalting inositol polyphosphates by dialysis. 10: The Ins(1,4,5)P3 receptor. 11: Purification and assay of inositol hexakisphosphate kinase and diphosphoinositol pentakisphosphate kinase. 12: Phosphoinositide 3-kinase
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