Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual
著者
書誌事項
Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual
Cold Spring Harbor Laboratory Press, c1998
1997 ed
大学図書館所蔵 全20件
内容説明・目次
内容説明
This edition of the Cold Spring Harbor Laboratory Press's yeast genetics course manual incorporates elements from the 1994 and 1990 publications. It also contains information on additional techniques such as mitotic recombination and random spore analysis, cytoduction, karyogamy, telomeric silencing, transformation techniques, and isolation of genomic DNA.
目次
- Genetic nomenclature. Looking at yeast cells. Experiments: isolation and characterization of auxotrophic, temperature-sensitive, and UV-sensitive mutants
- meiotic mapping
- mitotic recombination and random spore analysis
- transformation of yeast
- cytoduction and karyogamy
- gene replacement
- isolation of ras2 suppressors
- manipulating cell types
- isolation of suppressors of telomeric silencing by insertional shuttle mutagenesis
- lacZ gene fusion expression in yeast
- immunofluorescent staining of yeast cells. Techniques and protocols: super-efficient transformation of yeast with lithium acetate/SS-DNA/PEG
- other yeast transformation methods
- yeast colony hybridization
- yeast DNA isolations
- yeast protein extracts
- yeast RNA isolation
- northern analysis - formaldehyde agarose gel, blotting, and hybridization to filters
- alkaline southern blotting procedure
- scoring killer factor
- hydroxylamine mutagenesis of plasmid DNA
- assay of galactosidase in yeast
- transformation of bacteria (CaC12 method)
- plate assay for carboxypeptidase
- western blotting protein extracts
- random spore analysis
- yeast vital strains
- yeast immunofluorescence with antibodies
- actin staining in fixed cells
- PCR analysis of genotype
- quick e coli plasmid DNA mini-prep. Appendices: media
- stock preservation
- yeast genetic map
- grids
- electrophoretic karotypes of strains for southern blot mapping
- strains
- counting yeast cells with a standard hemocytometer chamber.
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