PRINS and in situ PCR protocols
著者
書誌事項
PRINS and in situ PCR protocols
（Methods in molecular biology / John M. Walker, series editor, 71）
Humana Press, c1997
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Cutting edge researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify or extend complimentary sequences in situ. Through these procedures often invented by the authors the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques suitable for both novice and experienced researchers have rapidly become indispensable for many clinical diagnostic procedures, whether in quantification of chromosomes in the identification of aneuploidy for prenatal diagnosis, or in the identification of viral infection in the early stages, or of rare messenger RNAs present in cells.
目次
Oligonucleotide PRINS DNA Synthesis John R. Gosden and Diane Lawson. Chromosome-Specific PRINS Jean-Paul Charlieu and Franck Pellestor. Bright-Field Microscopic Detection of Oligonucleotide PRINS-Labeled DNA in Chromosome Preparations Ernst J. M. Speel Daine Lawson Frans C. S. Ramaekers John R. Gosden and Anton H. N. Hopman. Analysis of Sperm Aneuploidy by PRINS Franck Pellestor and Jean-Paul Charlieu. PRINS DNA Synthesis on Frozen Tissue Sections Ernst J. M. Speel Diane Lawson Frans C. S. Ramaekers John R. Gosden and Anton H. N. Hopman. Multiple Sequential Oligonucleotide Primed In Situ DNA Synthesis (MULTI-PRINS) John R. Gosden and Diane Lawson. Oligonucleotide PRINS DNA Synthesis on Extended Chromatin Preparations Yoshiro Shibasaki and John R. Gosden. Combined Immunocytochemistry and PRINS DNA Synthesis for Simultaneous Detection of Phenotypic and Genomic Parameters in Cells Ernst J. M. Speel Diane Lawson Frans C. S. Ramaekers John R. Gosden and Anton H. N. Hopman. Chromosomal PRINS DNA Labeling Combined with Indirect Immunocytochemistry Arthur R. Mitchell. Direct In Situ Single-Copy (DISC)-PCR Deryl Troyer Hongyan Xie and Jie Hu. In Situ PCR to Cells and to Wax Sections: An Alternative to In Situ Hybridization for the Detection of Low Copies of Human Papillomavirus and Cytomegalovirus DNA Christiane Mougin Jean Marc Didier Dominique Bettinger Laurence Madoz Sylviane Coumes-Marquet and Michel Lab. Reverse Transcriptase PCR In Situ on Cryopreserved Tissue Sections Fred J. Hendler Alice Shum-Siu Briana W. Heniford Mohammed El Mekki Ahmed and V. J. Patel. Flow Cytometry Analysis of an In Situ PCR for the Detection of Human Immunodeficiency Virus Type-1 (HIV-1) Proviral DNA David E. Gibellini Maria Carla Re Giuliano Furlini and Michele La Placa. In Situ PCR Amplification of Intracellular mRNA Raymond H. Chen and Susan V. Fuggle. Localized In Situ Amplification (LISA): PCR Amplification of Nucleic Acid Sequences in Tissue Sections Gregory J. Tsongalis. In Situ PCR: An Overview Aidan A. Long and Paul Komminoth. Index.
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