Mutation detection

著者

• Cotton, Richard G. H.

書誌事項

Mutation detection

Richard G. H. Cotton

Oxford University Press, 1997

• pbk.

注記

Includes bibliographical references and index

内容説明・目次

巻冊次

ISBN 9780198548881

内容説明

Cancer and the mechanisms of its development are receiving increasing research interest. New genes are being isolated almost daily. Fundamental to those studies is the ability to detect mutations. Mutation detection is time consuming and expensive and thus, should be undertaken with a thorough knowledge of the alternative methods. This book aims to critically review the field, refer to methodology and protocols and give illustrative examples as well as put the whole emerging field in context including ethics.

目次

1.: Introduction. 2.: Sequencing Methods. 3.: Scanning Methods. 3.1: Physical Methods. 3.1.1.: Denaturing Gradient Gel Electrophoresis. 3.1.2.: Single Strand Conformational Polymorphism. 3.1.3.: Heteroduplex. 3.2.: Mismatch Detection/Cleavage. 3.2.1.: Ribonuclease. 3.2.2.: Chemical Cleavage. 3.2.3.: Carbodiimide. 3.2.3.: DNA endonucleases and binding proteins. 3.3.: Other. 4.1.: Allele Specific Oligonucleotide. 4.2.: Ligase and LCR. 4.3.: Allele Specific Amplification. 4.4.: Primer Extension. 4.5.: Artificial Introduction of Restriction Sites. 4.6.: Restriction Enzymes. 5.: Associated Methodology. 5.1.: Southern Blots. 5.2.: PCR. n 5.3.Illegitimate Transcription. 5.4.: Gene Specific Strategies. 6.: Current Weaknesses and Future Requirements

巻冊次

pbk. ISBN 9780198548898

内容説明

Cancer and the mechanisms of its development are receiving increasing research interest. New genes are being isolated almost daily, Fundamental to those studies is the ability to detect mutations. Mutation detection is time consuming and expensive and thus, should be undertaken with a thorough knowledge of the alternative methods. This book aims to critically review the field, refer to methodology and protocols and give illustrative examples as well as put the whole emerging field in context including ethics.

目次

• 1. Introduction
• 2. Sequencing Methods
• 3. Scanning Methods
• 3.1 Physical Methods
• 3.1.1. Denaturing Gradient Gel Electrophoresis
• 3.1.2. Single Strand Conformational Polymorphism
• 3.1.3. Heteroduplex
• 3.2. Mismatch Detection/Cleavage
• 3.2.1. Ribonuclease
• 3.2.2. Chemical Cleavage
• 3.2.3. Carbodiimide
• 3.2.3. DNA endonucleases and binding proteins
• 3.3. Other
• 4.1. Allele Specific Oligonucleotide
• 4.2. Ligase and LCR
• 4.3. Allele Specific Amplification
• 4.4. Primer Extension
• 4.5. Artificial Introduction of Restriction Sites
• 4.6. Restriction Enzymes
• 5. Associated Methodology
• 5.1. Southern Blots
• 5.2. PCR
• ILLEGITIMATE TRANSCRIPTION
• 5.4. Gene Specific Strategies
• 6. Current Weaknesses and Future Requirements

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BA34855069
• ISBN:
  • 0198548893
  • 0198548885
• LCCN: 96008969
• 出版国コード: uk
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Oxford ; New York
• ページ数/冊数: xvii, 198 p.
• 大きさ: 24 cm
• 分類:
  • LCC : QH442
  • DC20 : 574.87/328
• 件名:
  • LCSH : Molecular genetics
  • LCSH : Mutation (Biology)