Nitric oxide protocols
著者
書誌事項
Nitric oxide protocols
（Methods in molecular biology / John M. Walker, series editor, 100）
Humana, c1998
- :comb.
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
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ISBN 9780896034709
内容説明
Michael Titheradge provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of NO synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.
目次
Enzymology of Nitric Oxide Synthases. Purification of the Inducible Nitric Oxide Synthase. Purification of the Constitutive Nitric Oxide Synthase. Cloning and Expression of Human Inducible Nitric Oxide Synthase. Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System. Assay of NOS Activity by the Measurement of Conversion of Oxyhemoglobin to Methemoglobin by NO. Measurement of NOS Activity by Conversion of Radiolabeled Arginine to Citrulline Using Ion-Exchange Separation. Radiochemical Measurement of NOS Activity by Conversion of [14C]l-Arginine to Citrulline Using HPLC Separation. The Enzymatic Measurement of Nitrate and Nitrite. Determination of NOS Activity Using Cyclic-GMP Formation. Determination of NO with a Clark-Type Electrode. The Measurement of NO in Biological Systems Using Chemiluminescence. Measurement of NO Using Electron Paramagnetic Resonance. Measurement of eNOS and iNOS mRNA Expression Using Reverse Transcription Polymerase Chain Reaction. Measurement of NOS mRNA by Northern Blotting and the Ribonuclease-Protection Assay. Determination of NOS Isoforms by Western-Blot Analysis. Immunohistochemical Localization of NOS Isoforms. A Practical Protocol for the Demonstration of NOS Using In Situ Hybridization. Use of NO Donors in Biological Systems. Making and Working with Peroxynitrite. The Use of NO Gas in Biological Systems. A Microtiter-Plate Assay of Human NOS Isoforms. Use of Arginine Analogs as Inhibitors of Nitric Oxide Synthase in Rat-Aortic Rings. Measurement of Biopterin and the Use of Inhibitors of Biopterin. Biosynthesis. A Sensitive Assay for the Enzymatic Activity of GTP Cyclohydrolase. Simultaneous Measurement of Mitochondrial Function and NO. Release of NO from Donor Compounds: A Mathematical Model for Calculation of NO Concentrations in the Presence of Oxygen. The Determination of Nitrotyrosine Residues in Proteins. Measurement of DNA Damage Using the Comet Assay. Methods for the Study of NO-Induced Apoptosis in Cultured Cells. Index.
- 巻冊次
-
:comb. ISBN 9780896035379
内容説明
Michael Titheradge provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of NO synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.
目次
Enzymology of Nitric Oxide Synthases Benjamin Hemmens and Bernd Mayer. Purification of the Inducible Nitric Oxide Synthase Edward P. Garvey. Purification of the Constitutive Nitric Oxide Synthase Edward P. Garvey. Cloning and Expression of Human Inducible Nitric Oxide Synthase Mark L. Johnson, Richard A. Shapiro, and Timothy R. Billiar. Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System Ian G. Charles, Neale Foxwell, and Ann Chubb. Assay of NOS Activity by the Measurement of Conversion of Oxyhemoglobin to Methemoglobin by NO Mark Salter and Richard G. Knowles. Measurement of NOS Activity by Conversion of Radiolabeled Arginine to Citrulline Using Ion-Exchange Separation Richard G. Knowles and Mark Salter. Radiochemical Measurement of NOS Activity by Conversion of [14C]L-Arginine to Citrulline Using HPLC Separation James M. Cunningham and Richard C. Rayne. The Enzymatic Measurement of Nitrate and Nitrite Michael A. Titheradge. Determination of NOS Activity Using Cyclic-GMP Formation Suzanne G. Laychock. Determination of NO with a Clark-Type Electrode Kurt Schmidt and Bernd Mayer. The Measurement of NO in Biological Systems Using Chemiluminescence Evangelos D. Michelakis and Stephen L. Archer. Measurement of NO Using Electron Paramagnetic Resonance S. Tsuyoshi Ohnishi. Measurement of eNOS and iNOS mRNA Expression Using Reverse Transcription Polymerase Chain Reaction Norbert Reiling, Artur J. Ulmer, and Sunna Hauschildt. Measurement of NOS mRNA by Northern Blotting and the Ribonuclease-Protection Assay Paloma Martin-Sanz and Lisardo Bosca. Determination of NOS Isoforms by Western-Blot Analysis Fiona S. Smith and Michael A. Titheradge. Immunohistochemical Localization of NOS Isoforms Victoria Cattel and Karen Mosley. A Practical Protocol for the Demonstration of NOS Using In Situ Hybridization G. Michael Taylor and H. Terence Cook. Use of NO Donors in Biological Systems Jayne M. Tullettand Daryl D. Rees. Making and Working with Peroxynitrite C. Roger White, John P. Crow, Nathan Spear, Steven Thomas, Rakesh Patel, Irene Green, Joseph Beckman, and Victor Darley-Usmar. The Use of NO Gas in Biological Systems Neil Hogg and B. Kalyanaraman. A Microtiter-Plate Assay of Human NOS Isoforms John Dawson and Richard G. Knowles. Use of Arginine Analogs as Inhibitors of Nitric Oxide Synthase in Rat-Aortic Rings Rachel J. Russell. Measurement of Biopterin and the Use of Inhibitors of Biopterin Biosynthesis Kzuyuki Hatakeyama. A Sensitive Assay for the Enzymatic Activity of GTP Cyclohydrolase IKazuyuki Hatakeyama and Toshie Yoneyama. Simultaneous Measurement of Mitochondrial Function and NO I gnacio Lizasoain and Maria A. Moro. Release of NO from Donor Compounds: A Mathematical Model for Calculation of NO Concentrations in the Presence of Oxygen Kurt Schmidt, Wolfgang Desch, Peter Klatt, Walter R. Kukovetz, and Bernd Mayer. The Determination of Nitrotyrosine Residues in Proteins Andrew J. Gow, Molly McClelland, Sarah E. Garner, Stuart Malcom, and Harry Ischiropoulos. Measurement of DNA Damage Using the Comet Assay Steven Thomas, Michael H. L. Green, Jillian E. Lowe, and Irene C. Green. Methods for the Study of NO-Induced Apoptosis in Cultured Cells Anne C. Loweth and Noel G. Morgan. Index.
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