Mutation detection : a practical approach
Author(s)
Bibliographic Information
Mutation detection : a practical approach
（The practical approach series, 188）
IRL Press at Oxford University Press, c1998
- : hbk
- : pbk
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Note
Includes bibliographical references and index
Description and Table of Contents
- Volume
-
: pbk ISBN 9780199636563
Description
Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnbosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading
researchers in the field.
Table of Contents
- 1. Single strand conformation polymorphism analysis
- 2. Single-stranded conformation polymorphism and heteroduplex analysis
- 3. Comprehensive mutation detection with denaturing gradient gel electrophoresis
- 4. Cleavage using RNase to detect mutations
- 5. Cleavage of mismatched bases using chemical reagents
- 6. Mutation detection using T4 endonuclease VII
- 7. Detection of mutations by hybridization with sequence-specific oligonucleotide probes
- 8. DNA detection and sequence distinction through oligonucleotide ligation
- 9. Detection of sequence variation using primer extension
- 10. Selective amplification of specific alleles
- 11. The protein truncation text (PTT)
- 12. Functional assay of the p53 tumour suppressor gene
- 13. Advances in direct DNA sequencing for mutation scanning
- Volume
-
: hbk ISBN 9780199636570
Description
Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.
Table of Contents
1: Single strand conformation polymorphism analysis. 2: Single-stranded conformation polymorphism and heteroduplex analysis. 3: Comprehensive mutation detection with denaturing gradient gel electrophoresis. 4: Cleavage using RNase to detect mutations. 5: Cleavage of mismatched bases using chemical reagents. 6: Mutation detection using T4 endonuclease VII. 7: Detection of mutations by hybridization with sequence-specific oligonucleotide probes. 8: DNA detection and sequence distinction through oligonucleotide ligation. 9: Detection of sequence variation using primer extension. 10: Selective amplification of specific alleles. 11: The protein truncation text (PTT). 12: Functional assay of the p53 tumour suppressor gene. 13: Advances in direct DNA sequencing for mutation scanning
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