Immunochemical protocols

著者

• Pound, John D.

書誌事項

Immunochemical protocols

edited by John D. Pound

（Methods in molecular biology / John M. Walker, series editor, 80）

Humana Press, c1998

2nd ed

• : alk. paper
• : comb : alk. paper

注記

Includes bibliographical references and index

内容説明・目次

巻冊次

: comb : alk. paper ISBN 9780896033887

内容説明

This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including conventional and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.

目次

Production of Polyclonal Antisera,Jonathan A. Green and Margaret M. Manson. Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen,Monique Diano, Andre Le Bivic, and Michel Hirn. Production and Characterization of Antibodies Against Synthetic Peptides, David C. Hancock and Gerard I. Evan. Preparation and Testing of MAbs to Recombinant Proteins, Christopher J. Dean. A Simple Hollow-Fiber Bioreactor for the 'In-House' Production of Monoclonal Antibodies. Margaret Goodall. Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase,Yveline Frobert and Jacques Grassi. Synthesis of Peptides for Use as Immunogens David C. Hancock, Nicola J. O'Reilly, and Gerard I. Evan. Use of Proteins Blotted to Polyvinylidene Difluoride Membranes as Immunogens, Boguslaw Szewczyk and Donald F. Summers. Purification of Glycoproteins and Their Use as Immunogens, Boguslaw Szewczyk and Donald F. Summers.IgG Purification, Mark Page and Robin Thorpe. Purification of Monoclonal Antibodies, Mark Page and Robin Thorpe.Production of Bispecific and Trispecific F(ab)2 and F(ab)3 Antibody Derivatives, Ruth R. French. Preparation of Cytotoxic Antibody & Toxin Conjugates, Alan J. Cumber and Edward J. Wawrzynczak. Immunoafinity Purification and Quantification of Antibody-Toxin Conjugates Edward J. Wawrzynczak and Alan J. Cumber 15 Competitive ELISA Kittima Makarananda, Lucinda R. Weir, and Gordon E. Neal 155 16 Epitope Mapping Glenn E. Morris 161 17 Coupling of Antibodies with Biotin Rosaria P. Haugland and Wendy W. You 173 18 Preparation of Avidin Conjugates Rosaria P. Haugland and Mahesh K. Bhalgat 173 19 Enhanced Chemiluminescence Immunoassay Richard A. W. Stott 197 20 Nonradioactive Methods for Visualization of Protein Blots Michael Finney 207 21 Colloidal Gold Staining and Immunoprobingon the Same Western Blot Denise Egger and Kurt Bienzt 217 22 Erasure of Western Blots After Autoradiographic or Chemiluminescent Detection Scott H. Kaufmann and Udo Kellner 223 23 Colloidal Gold Staining and Immunodetection in 2D Protein Mapping Anthony H. V. Schapira 237 24 Enzyme_Antienzyme Method for Immunohistochemistry Michael G. Ormerod, Elizabeth Philp, and Susanne F. Imrie 243 25 Immunohistochemical Detection of Bromodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic Studies Jonathan A. Green, Richard E. Edwards, and Margaret M. Manson 251 26 Cell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine George D. Wilson 255 27 Immunohistochemical Detection of Cells in the Division Cycle Using Antibodies to Proliferating Cell Nuclear Antigen (PCNA) Richard Edwards and Jerry Styles 267 28 Double Label Immunohistochemistry on Tissue Sections Using Alkaline Phosphatase and Peroxidase Conjugates Jonathan A. Green and Margaret M. Manson 271 29 Preparation of Colloidal Gold Probes David A. Hughes and Julian E. Beesley 275 30 Immunogold Probes in Light Microscopy David A. Hughes and Julian E. Beesley 283 31 Immunogold Probes in Electron Microscopy David J. P. Ferguson, David A. Hughes, and Julian E. Beesley 297 32 Electron Microscopic-Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species Kurt Bienz and Denise Egger 313 33 Flow Cytometric Analysis of Cell Surface Antigen Density R. Adrian Robins 319 34 Multiple Immunofluorescence Analysis of Cells Using Flow Cytometry R. Adrian Robins 337 35 Flow Cytometric Methods of Analyzing Apoptotic Cells Anne E. Milner, J. Michaela Levens, and Christopher D. Gregory 347 36 Flow Cytometric Detection of Proliferation-Associated Antigens, PCNA and Ki-67 George D. Wilson 355 37 Sorting of Human Peripheral Blood T-Cell Subsets Using Immunomagnetic Beads Eddie C. Y. Wang 365 38 Preparation and Use of Nonradioactive Hybridization Probes Victor T.-W. Chan 377 39 Cellular H

巻冊次

: alk. paper ISBN 9780896034938

内容説明

This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including conventional and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.

目次

Production of Polyclonal AntiseraRaising Polyclonal Antibodies Using Nitrocellulose-Bound AntigenProduction and Characterization of Antibodies Against Synthetic Peptides Preparation and Testing of MAbs to Recombinant Proteins A Simple Hollow-Fiber Bioreactor for the In-House Production of Monoclonal Antibodies. Screening of Monoclonal Antibodies Using Antigens Labeled with AcetylcholinesteraseSynthesis of Peptides for Use as Immunogens Use of Proteins Blotted to Polyvinylidene Difluoride Membranes as Immunogens Purification of Glycoproteins and Their Use as Immunogens .IgG PurificationPurification of Monoclonal Antibodies Production of Bispecific and Trispecific F(ab)2 and F(ab)3 Antibody DerivativesPreparation of Cytotoxic Antibody & Toxin Conjugates Immunoafunity Puriucation and Quantiucation of Antibody Toxin Conjugates Competitive ELISA Kittima Makarananda Epitope Mapping Coupling of Antibodies with Biotin Preparation of Avidin Conjugates Enhanced Chemiluminescence Immunoassay Nonradioactive Methods for Visualization of Protein Blots Colloidal Gold Staining and Immunoprobingon the Same Western BlotErasure of Western Blots After Autoradiographic or Chemiluminescent Detection Colloidal Gold Staining and Immunodetection in 2D Protein Mapping Enzyme Antienzyme Method for Immunohistochemistry Immunohistochemical Detection of Bromodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic StudiesCell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine Immunohistochemical Detection of Cells in the Division Cycle Using Antibodies to Proliferating Cell Nuclear Antigen (PCNA) Double Label Immunohistochemistry on Tissue Sections Using Alkaline Phosphatase and Peroxidase Conjugates Preparation of Colloidal Gold Probes Immunogold Probes in Light Microscopy Immunogold Probes in Electron Microscopy Electron Microscopic-Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species Flow Cytometric Analysis of Cell Surface Antigen Density Multiple Immunofluorescence Analysis of Cells Using Flow CytometryFlow Cytometric Methods of Analyzing Apoptotic Cells Flow Cytometric Detection of Proliferation-Associated Antigens PCNA and Ki-67 Sorting of Human Peripheral Blood T-Cell Subsets Using Immunomagnetic Beads Preparation and Use of Nonradioactive Hybridization Probes Cellular Human and Viral DNA Detection by Nonisotopic In Situ Hybridization Biotinylated Probes in Colony Hybridization Nonisotopic In Situ Hybridization Gene Mapping and Cytogenetics Expression of Recombinant Antibody Fusion Proteins in E. coli Expression of Antibody Fusion Proteins in Mammalian Cells Antibody Screening of Bacteriophage lgt-11 DNA Expression Libraries Display of Antibody Chains on Filamentous Bacteriophage Production of Human Fab Antibody Fragments from Phage Display LibrariesAntibodies from Phage Display Libraries as Immunochemical ReagentsIndex

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詳細情報

• NII書誌ID(NCID): BA36584009
• ISBN:
  • 0896034933
  • 0896033880
• LCCN: 97039695
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Totowa, N.J.
• ページ数/冊数: xiii, 508 p.
• 大きさ: 23 cm
• 分類:
  • LCC : QR183.6
  • DC21 : 616.07/56
• 件名:
  • LCSH : Immunochemistry -- Methodology
• 親書誌ID:
  • BA10794673