Chromatin : a practical approach
Author(s)
Bibliographic Information
Chromatin : a practical approach
（The practical approach series）
IRL Press at Oxford University Press, 1998
- :hbk
- :pbk
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Note
Includes bibliographical references and index
Description and Table of Contents
- Volume
-
:pbk ISBN 9780199635986
Description
Chromatin comprises the complex of nucleic acids and proteins that make up the chromosomes, and regulation of its structure is increasingly recognized as an important factor in gene expression. Researchers from related fields needing an introduction to the methodology of chromatin should find this book useful. It should also be of interest to experienced researchers.
Table of Contents
- 1. Isolation and fractionation of chromatin and linker histones
- 2. Extraction and separation of core histones and non-histone chromosomal proteins
- 3. Mapping chromatin structure in Drosophila
- 4. In vivo footprint and chromatin analysis by LMPCR
- 5. Analysis of nuclear scaffold attachment regions
- 6. Electron microscopy of chromatin
- 7. Counting nucleosome cores on circular DNA using topoisomerase I
- 8. In vitro reconstitution of chromatin and analysis of nucleosome positioning
- 9. Transcriptional and structural analysis of chromatin assembled in vivo
- 10. An assembly of chromatin and nuclear structures in Xenopus egg extracts
- Appendix: List of Suppliers
- Index
- Volume
-
:hbk ISBN 9780199635993
Description
Chromatin comprises the complex of nucleic acids and proteins that make up the chromosomes, and regulation of its structure is increasingly recognized as an important factor in gene expression. Researchers from related fields needing an introduction to the methodology of chromatin should find this book useful. It should also be of interest to experienced researchers.
Table of Contents
1: Isolation and fractionation of chromatin and linker histones. 2: Extraction and separation of core histones and non-histone chromosomal proteins. 3: Mapping chromatin structure in Drosophila. 4: In vivo footprint and chromatin analysis by LMPCR. 5: Analysis of nuclear scaffold attachment regions. 6: Electron microscopy of chromatin. 7: Counting nucleosome cores on circular DNA using topoisomerase I. 8: In vitro reconstitution of chromatin and analysis of nucleosome positioning. 9: Transcriptional and structural analysis of chromatin assembled in vivo. 10: An assembly of chromatin and nuclear structures in Xenopus egg extracts. Appendix: List of Suppliers. Index
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