Methods in chemical ecology

書誌事項

Methods in chemical ecology

Chapman & Hall, 1998

  • v. 1 : alk. paper
  • v. 2 : alk. paper

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注記

Includes bibliographical references and index

収録内容

  • v. 1. Chemical methods / edited by Jocelyn G. Millar, Kenneth F. Haynes
  • v. 2. Bioassay methods / edited by Kenneth F. Haynes, Jocelyn G. Millar

内容説明・目次

巻冊次

v. 2 : alk. paper ISBN 9780412080418

内容説明

Identification of chemicals that affect the naturally occurring interactions be tween organisms requires sophisticated chemical techniques, such as those docu mented in volume 1, in combination with effective bioassays. Without an effective bioassay, the identification becomes akin to looking for a needle in a haystack, but without any idea of what a needle looks like. To a large extent serniochemical identifications must be driven by bioassays. The design of bioassays for use in chemical ecology is governed by the sometimes conflicting objectives of ecological relevance and the need for simplic ity. Bioassay design should be based on observations of the interactions between organisms in their natural context, a theme that appears throughout this volume. As a result, this volume is as much about ecology and behavior as it is about specific methods. It is impossible to design a relevant bioassay, whether it is simple or complex, without understanding at least the fundamentals of how chemical cues or signals mediate the interaction in nature. Thus, the development of bioassay methods must be driven by an understanding of ecology and a knowledge of the natural history of the organisms under study. Given such an understanding, it is often possible to design assays that are both ecologically relevant and easy to perform.

目次

  • Preface. 1. Bioassays with Marine Microorganisms
  • K.M. Jenkins, et al. 2. Bioassays with Marine and Freshwater Macroorganisms
  • M.E. Hay, et al. 3. Bioassay Methods for Fungi and Oomycetes
  • J.L. Kerwin, M.J. Semon. 4. Bioassays for Allelopathy in Terrestrial Plants
  • J.T. Romeo, J.D. Weidenhamer. 5. Bioassay Methods with Terrestrial Invertebrates
  • J.D. Hare. 6. Bioassay Methods for Amphibians and Reptiles
  • R.T. Mason, et al . 7. Bioassays for Mammals and Birds
  • D.L. Nolte, J.R. Mason. Index.
巻冊次

v. 1 : alk. paper ISBN 9780412080715

内容説明

A working definition of the discipline of chemical ecology might be "the study of the structure, function, origin, and significance of naturally occurring compounds that mediate inter-and intraspecific interactions between organisms. " In particular, chemical ecology focuses on determining the role of semiochemicals and related compounds in their natural contexts. Thus, chemical ecology is distinct from disciplines such as pharmacology, in which compounds are screened for uses outside their natural context, for example in the screening of natural products for use as drugs. Superficially, many of the methods used in the various branches of natural products chemistry, such as pharmacology and chemical ecology, are very similar, but each branch has developed its own set of specialized methods for dealing with the problems characteristic of that discipline. For example, in chemical ecology, many semiochemicals are isolated and identified using only a few micrograms or less of material. Although the same general chromatographic and spectroscopic techniques are used as would be used with the identification of most organic compounds, specialized techniques have been developed for handling these very small quantities, allowing the maximum amount of informa tion to be recovered from the minimum amount of sample. These micro scale techniques, and the problems unique to working with very small amounts of sample, are rarely covered in detail in reference books on the isolation and identification of biologically active natural chemicals.

目次

  • Preface. 1. Preparation, Cleanup, and Preliminary Fractionation of Extracts
  • J.G. Millar, J.J. Sims. 2. Liquid Chromatography
  • J.G. Millar. 3. Analytical and Preparative Gas Chromatography
  • R.R. Heath, B.D. Dueben. 4. Mass Spectrometry
  • F.X. Webster, J.G. MIllar, D.J. Kiemle. 5. Structure Elucidation by NMR
  • F.X. Webster, D.J. Kiemle. 6. Infrared and Ultraviolet Spectroscopy Techniques
  • W.S. Leal. 7. Microchemical Techniques
  • A.B. Attygalle. 8. Separation of Enantiomers and Determination of Absolute Configuration
  • K. Mori. 9. Electrophysiological Methods
  • L.B. Bjostad. Appendix: List of Names and Addresses of Suppliers. Index.

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