PCR protocols in molecular toxicology
著者
書誌事項
PCR protocols in molecular toxicology
（CRC Press methods in the life sciences, CRC Press methods in toxicology）
CRC Press, c1998
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:
Quantitative reverse transcriptase PCR and methods to examine gene expression
Differential display cloning
Cloning and library screening by PCR
Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes
Basic, non-PCR based molecular biology methods
PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.
目次
Chapter 1. The Basics of the Polymerase Chain Reaction -- /William B. Mattes -- Chapter 2. Analysis of Gene Expression -- /John P. Vanden Heuoel -- Chapter 3. Differential Display PCR -- /J. Christopher Corton -- Chapter 4 . Cloning by PCR -- /John P. Vanden Heuuel -- Chapter 5. Genotype Analysis -- /Douglas A. Bell and Gary Pittman -- Chapter 6 . General Molecular Biology Techniques -- /John P. Vanden Heuvel -- Appendix I - List of Suppliers -- Appendix II - Companies, Online Catalogs, and Internet Resources -- Index.
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