Vectors : cloning applications
著者
書誌事項
Vectors : cloning applications
（Essential techniques series）
John Wiley, c1998
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注記
"Published in association with BIOS Scientific Publishers Limited."
Includes bibliographical references and index
内容説明・目次
内容説明
Cloning vectors are small DNA molecules which can have DNA fragments introduced in vitro using restriction enzymes and DNA ligases. Vectors: Cloning Applications is a handy laboratory manual which allows quick and easy access to the key protocols required by those working with vectors. This volume guides readers towards the best choice of method, conditions, equipment and reagents and provides them with trouble shooting tips to help if and when a technique runs into problems. The manual provides comprehensive coverage of vectors, including the commercially available ones which are difficult to trace through current literature and offers a different approach to an area which is central to many of the techniques used in molecular biology.
目次
- General cloning vectors
- "in vitro" transcription/translation vectors
- bacteriophage lambda vectors
- cosmid vectors
- cloning of PCR products
- manipulation of cloned DNA
- preparation and sizes of molecular size markers.
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