Practical plant virology : protocols and exercises

書誌事項

Practical plant virology : protocols and exercises

Jeanne Dijkstra, Cees P. de Jager

(Springer lab manual)

Springer, c1998

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Unlike most pathogenic organisms, viruses require a special approach to establish their presence in a diseased plant, since they are not visible, even with a light microscope. Due to the tremendous damage of crop plants caused by viruses various methods and techniques have been developed to enable the investigation of these pathogens. A variety of protocols for determining the properties and identity of a virus or its behaviour in an infected plant are described in this manual. For teaching purposes, a number of class exercises have been added. The protocols have been selected in such a way as to be useful for plant viruses in both tropical and non-tropical parts of the world.

目次

  • Parts: Virus Inoculation
  • Symptomatology
  • Infectivity assays
  • Infectivity of virus in crude sap
  • Transmission of viruses
  • Maintenance of virus isolates
  • Production of virus-free material from infected plants
  • Virus isolation and purification
  • Physico-chemical properties of virus particles
  • Properties of viral components
  • Serological techniques for detection and identification of viruses and viral products
  • Electron microscope and light microscope serology
  • Detection of viral RNA
  • Diagnosis of a disease and identification of the causal virus(es) The complete table of contents can be found on the Internet. http://www springer.de

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