Practical plant virology : protocols and exercises
著者
書誌事項
Practical plant virology : protocols and exercises
(Springer lab manual)
Springer, c1998
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Unlike most pathogenic organisms, viruses require a special approach to establish their presence in a diseased plant, since they are not visible, even with a light microscope. Due to the tremendous damage of crop plants caused by viruses various methods and techniques have been developed to enable the investigation of these pathogens. A variety of protocols for determining the properties and identity of a virus or its behaviour in an infected plant are described in this manual. For teaching purposes, a number of class exercises have been added. The protocols have been selected in such a way as to be useful for plant viruses in both tropical and non-tropical parts of the world.
目次
- Parts: Virus Inoculation
- Symptomatology
- Infectivity assays
- Infectivity of virus in crude sap
- Transmission of viruses
- Maintenance of virus isolates
- Production of virus-free material from infected plants
- Virus isolation and purification
- Physico-chemical properties of virus particles
- Properties of viral components
- Serological techniques for detection and identification of viruses and viral products
- Electron microscope and light microscope serology
- Detection of viral RNA
- Diagnosis of a disease and identification of the causal virus(es) The complete table of contents can be found on the Internet. http://www springer.de
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