Receptor signal transduction protocols

著者

• Challiss, R. A. J. (R. A. John)

書誌事項

Receptor signal transduction protocols

edited by R.A.J. Challiss

（Methods in molecular biology / John M. Walker, series editor, 83）

Humana Press, c1997

• comb
• hard

注記

Includes bibliographical references and index

内容説明・目次

巻冊次

comb ISBN 9780896034181

内容説明

An outstanding collection of state-of-the-art methods from leading experts for interrogating ligand-receptor and receptor-effector interactions. The methods emphasize the regulation of G protein-coupled receptors and focus on receptor characterization, methods for investigating how receptors couple to G proteins, and approaches to studying how receptor expression and function is regulated. The easily reproducible protocols include chimeric receptor construction, antibody generation, transient and stable transfection methods, in situ hybridization, as well as immunocytochemistry, functional assays of G protein function, antisense methods for defining receptor-effector coupling pathways, and many more.

目次

Methods for Characterizing Receptors. Radioligand-Binding Methods for Membrane Preparations and Intact Cells Mary Keen. Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding Mark E. Olah and Gary L. Stiles. Approaches to the Stable Transfection of G Protein-Coupled Receptors Andrea Townsend-Nicholson. Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels Hartmut L?ddens and Esa R. Korpi. The Generation of Receptor-Selective Antibodies John J. Mackrill. In Situ Hybridization Judith C.W. Mak and Peter J. Barnes. Immunocytochemical Methods for Investigating Receptor Localization Maria I. Fonseca and R. Dale Brown. Methods for studying receptor coupling mechanisms. Measurement of Agonist-Stimulated [35S]GTPgS Binding to Cell Membranes Sebastian Lazareno. Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTPgS Binding Laura J. Sim Dana E. Selley and Steven R. Childers. Agonist-Induced High-Affinity GTP Hydrolysis As an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes Yuji Odagaki and Kjell Fuxe. Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling Ethan S. Burstein Tracy A. Spalding and Mark R. Brann. Identification and Quantitation of G Protein a-Subunits Ian Mullaney and Graeme Milligan. Covalent Modification of G Proteins by Affinity Labeling Martin Hohenegger Michael Freissmuth and Christian Nanoff. Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection Stephen R. Ikeda. Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identity G Protein Heterotrimers Activating Phospholipase C Frank Kalkbrenner Edgar Dippel Matthias Schroff Burghardt Wittig and G?nter Schultz. Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons Fe C. Abogadie Yvonne Vallis Noel J. Buckley and Malcolm P. Caulfield. Methods for investigating the regulation of receptor function. Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation Andrew B. Tobin. Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation Alison W. Gagnon and Eamonn Kelly. Radioligand Binding Measurements of Receptor Sequestration in Intact and Permeabilized Cells Diana M. Slowiejko and Stephen K. Fisher. Regulation of Receptor Expression: Analysis of Receptor mRNA and Gene Transcription Philip A. Iredale Maura E. Charlton John D. Alvaro and Ronald S. Duman. Index.

巻冊次

hard ISBN 9780896034952

内容説明

Gaining an understanding of the mechanisms by which cells process and respond to extracellular cues has become a major goal in many areas of bi- ogy and has attracted the attentions of almost every traditional discipline within the biological sciences. At the heart of these divergent endeavors are common methods that can aid biochemists, physiologists, and pharmacologists in ta- ling the specific questions addressed by their research. In Receptor Signal Transduction Protocols, a diverse array of meth- ologies employed to interrogate ligand--receptor and receptor-effector int- actions are described by authors who have devised and successfully applied them. The authors blend excellent descriptions and applications of fairly well established methodologies with new technologies at the cutting-edge of signal transduction research and as such I hope the present volume will complement and extend a previous excellent volume in this series edited by David Kendall and Stephen Hill (Methods Molecular Biology, vol. 41, Signal Transd- tion Protocols).

目次

Methods for Characterizing Receptors. Radioligand-Binding Methods for Membrane Preparations and Intact Cells Mary Keen Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding Mark E. Olah and Gary L. Stiles Approaches to the Stable Transfection of G Protein-Coupled Receptors Andrea Townsend-Nicholson Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels Hartmut Luddens and Esa R. Korpi The Generation of Receptor-Selective Antibodies John J. Mackrill In Situ Hybridization Judith C.W. Mak and Peter J. Barnes Immunocytochemical Methods for Investigating Receptor Localization Maria I. Fonseca and R. Dale Brown Methods for Studying Receptor Coupling Mechanisms. Measurement of Agonist-Stimulated [35S]GTPgS Binding to Cell Membranes Sebastian Lazareno Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTPgS Binding Laura J. Sim, Dana E. Selley, and Steven R. Childers Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes Yuji Odagaki and Kjell Fuxe Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling Ethan S. Burstein, Tracy A. Spalding, and Mark R. Brann Identification and Quantitation of G Protein a-Subunits Ian Mullaney and Graeme Milligan Covalent Modification of G Proteins by Affinity Labeling Martin Hohenegger, Michael Freissmuth, and Christian Nanoff Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection Stephen R. Ikeda Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identity G Protein Heterotrimers Activating PhospholipaseC Frank Kalkbrenner, Edgar Dippel, Matthias Schroff, Burghardt Wittig, and Gunter Schultz Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons Fe C. Abogadie, Yvonne Vallis, Noel J. Buckley, and Malcolm P. Caulfield Methods for Investigating the Regulation of Receptor Function Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation Andrew B. Tobin Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation Alison W. Gagnon and Eamonn Kelly Radioligand Binding Measurements of Receptor Sequestration in Intact and Permeabilized Cells Diana M. Slowiejko and Stephen K. Fisher Regulation of Receptor Expression: Analysis of Receptor mRNA and Gene Transcription Philip A. Iredale, Maura E. Charlton, John D. Alvaro, and Ronald S. Duman Index.

「Nielsen BookData」 より

• NII書誌ID(NCID): BA37492926
• ISBN:
  • 0896034186
  • 089603495X
• LCCN: 97012424
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Totowa, N.J.
• ページ数/冊数: xi, 275 p.
• 大きさ: 24 cm
• 分類:
  • LCC : QH603.C43
  • NLM : W1 ME9616J v.83 1997
  • NLM : QH 601 R2954 1997
  • DC21 : 572/.69
• 件名:
  • LCSH : Cell receptors -- Research -- Methodology
  • LCSH : Cellular signal transduction -- Research -- Methodology
  • MESH : Signal Transduction -- physiology
• 親書誌ID:
  • BA10794673