Enzyme assays : essential data
著者
書誌事項
Enzyme assays : essential data
(Essential data series)
Wiley, 1998
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Enzyme Assays S. Gul, S.K. Sreedharan and K. Brocklehurst The development of an assay procedure by which the course of a particular enzyme--catalyzed reaction may be monitored is an essential preliminary step in virtually all experimental studies on the enzyme. This book summarizes the key principles underlying enzyme assays, including assay design and the evaluation of experimental data. It provides details of and references to the major physico--chemical techniques commonly used in enzymology. Useful ancillary data are also included on subjects such as active site titration, reagent kits and buffers. The Essential Data series provides rapid access to the core data required by researchers on a daily basis, in convenient pocket--sized volumes.
目次
Enzyme Assays: An Overview. Evaluation and Some General Applications of Experimental Data. Individual Assay Techniques: Principles, Methods and Examples. Active Site Titration. Reagent Kits for Determination of Enzyme Activities. Buffers, Protein Denaturants, Acids and Bases and Radioisotopes. Main Manufacturers and Suppliers. References. Index.
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