DNA transfer to cultured cells
著者
書誌事項
DNA transfer to cultured cells
(Culture of specialized cells)
Wiley-Liss, c1998
大学図書館所蔵 全6件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
注記
Includes bibliographical references and index
"A John Wiley & Sons, Inc., publication."
内容説明・目次
内容説明
DNA transfer to cultured cells
Edited by Katya Ravid and R. Ian Freshney
Rapid advances in DNA transfer technology have transformed many disciplines, ranging from molecular genetics to biotechnology. Scientists now have the means to introduce copies of genes into different cell types, then detect the expression of these genes in the cell. It is now possible to regulate cell growth that may lead to cancer, develop new biopharmaceuticals, and apply knowledge about the role of genes in cell processes to basic research in molecular genetics.
DNA Transfer to Cultured Cells is the first quick reference to all of the established techniques for the transfer of genetic material to cells in vitro. Featuring contributions by leading researchers in the field, this detailed guide walks the reader through a variety of DNA transfer methods, describes their application to specific cell types, and integrates aspects of molecular biology with tissue culture. Offering overviews and detailed protocols for the techniques under discussion in each of its sections, this book covers an exceptionally broad array of topics, including:
* Viral infection
* Electroporation
* Phosphate precipitation
* DEAE Dextran
* Liposomes
* Yeast artificial chromosomes (YACs)
* Whole chromosome transfer
* Enhanced expression.
Special sections at the end of each chapter list suppliers for necessary reagents and materials. This easy-to-use, self-contained guide addresses key developments of recent years as well as emerging trends in DNA transfer. For practical applications in cell biology, genetics, heredity, biotechnology, or evolution, DNA Transfer to Cultured Cells is a unique and unparalleled resource.
目次
Partial table of contents:
Production and Use of Retroviruses (G. Hicks, et al.).
Electroporation of DNA into Cultured Cell Lines (L. Cataldo, et al.).
Transfection of Rat Adipose Cells by Electroporation (M. Quon).
Calcium Phosphate Transfection (K. Conn. et al.).
Strontium Phosphate Transfection (R. Reddel, et al.).
DEAE- Dextran Transfection (J. Schwartz & R. Rosenberg).
Cationic Liposomes (V. Bichko).
Transfection of Mammalian Cells with Yeast Artificial Chromosomes. (W. Strauss).
Mapping Human Senescence Genes Using Microcell-Mediated Chromosome Transfer (R. Newbold & A. Cuthbert).
Methotrexate and DHFR Amplification (M. Ricketts).
List of Suppliers.
Index.
「Nielsen BookData」 より