Biomedical electron microscopy : illustrated methods and interpretations
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Bibliographic Information
Biomedical electron microscopy : illustrated methods and interpretations
Academic Press, c1999
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Includes bibliographical references and index
Description and Table of Contents
Description
This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis.
Table of Contents
Micrograph InterpretationFixativesFixative VehicleFixative ApplicationDehydration and EmbeddingFreezing and Freeze-SubstitutionSupport FilmsUltramicrotomySection-StainingMicroscopyImage RecordingPhotographic and Digital PrintingNegative StainingAutoradiographyCytochemistryImmunocytochemistryFreeze-Fracturing and ShadowingSampling and QuantitationImage Processing3D ReconstructionsAppendix: Practical Methods
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