Biocalorimetry : applications of calorimetry in the biological sciences
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Bibliographic Information
Biocalorimetry : applications of calorimetry in the biological sciences
J. Wiley, c1998
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Papers presented at a conference held at the St. Anne's College in Oxford, UK, Sept. 1996
Includes bibliographical references and index
Description and Table of Contents
Description
Biocalorimetry Applications of Calorimetry in the Biological Sciences Edited by John E. Ladbury, University College London, UK and Babur Z. Chowdhry, University of Greenwich, London, UK Biocalorimetry: Applications of Calorimetry in the Biological Sciences is the first book to bring together an overview of the two key methods in calorimetry, (isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC)), with a wide variety of biological applications, such as DNA-drug/protein interactions, protein-protein (ligand) interactions and protein denaturation studies. Written by a team of international researchers, it discusses the principles and practical application of these methods, including their advantages and disadvantages. This book will be of interest to researchers working in biochemistry, biophysics and molecular biology, particularly those in the pharmaceutical, food and biotechnology industries.
In addition, Biocalorimetry: Applications of Calorimetry in the Biological Sciences provides valuable insight for lecturers and students, (particularly final year undergraduates and postgraduates), for teaching purposes, as well as for understanding the theoretical basis and wider practical applications of calorimetry.
Table of Contents
INTRODUCTION TO ISOTHERMAL TITRATION CALORIMETRY. Thermodynamic Background to Isothermal Titration Calorimetry (M. Blandamer). Isothermal Titration Calorimetry of Biomolecules (J. Tame, et al.). DNA-DRUG INTERACTIONS. Isothermal Titration Calorimetry in the Study of DNA-Drug Interactions (I. Haq). The Thermodynamics of the Association of Amsacrine Derivatives and Netropsin-Amsacrine Combilexin to DNA Duplexes and to Chromatin (A. Taquet & C. Houssier). PHOSPHOLIPID-LIGAND INTERACTIONS. Phospholipid-Ligand Interactions (A. Blume). Thermodynamics of Hydrophobic and Steric Lipid/Additive Interactions (H. Heerklotz). PROTEIN-PROTEIN INTERACTIONS. Microcalorimetry of Protein-Protein Interactions (A. Cooper). Folding Energetics of a Heterodimetric Leucine Zipper (I. Jelesarov & H. Bosshard). Interaction of Ribonuclease S with Ligands from Random Peptide Libraries (D. Schultz, et al.). PROTEIN-DNA INTERACTIONS. Calorimetric Studies of Dehydration and Salt Effects in Protein-DNA Interactions (T. Lundb?ck & T. H?rd). Thermodynamic and Kinetic Analysis of RecA-DNA Interactions for Understanding the Recognition of Homologous DNA (F. Maraboeuf, et al.). INTRODUCTION TO DEFFERENTIAL SCANNING CALORIMETRY. Thermodynamic Background to Differential Scanning Calorimetry (S. Leharne & B. Chowdhry). Quantitative Analysis of Differential Scanning Colorimetric Data (D. Haynie). INSTRUMENTATION. An Ultrasensitive Scanning Calorimeter (V. Plotnikov, et al.). Design of a High-Throughput Microphysiometer (K. Verhaegen, et al.). PROTEIN DENATURATION STUDIES. Denaturation Studies of haFGF (D. Adamek, et al.). Relaxation Constants as a Preditor of Protein Stabilization (J. Anekwe, et al.). Domain Dynamics of the Bacillus subtilis Peripheral Preprotein translocase Subunit SecA (T. den Blaauwen & A. Driessen). Cooperative Structures within Glycosylated and Aglycosylated Mouse IgG2b (V. Tishchenko, et al.). Contributions of Free Cysteine Residues to the Stability of the Human Acidic Fibroblast Growth Factor (A. Popovic, et al.). Differential Scanning Calorimetry of Phosphoglycerate Kinase from the Hyperthermophilic Bacterium Thermotoga maritima (K. Zaiss, et al.). PROTEIN-LIGAND INTERACTIONS. The Effect of Cyclodextrins on Monomeric Protein Unfolding (S. Branchu, et al.). Interdomain Interactions in the Mannitol Permease of E. coli (W. Meijberg, et al.). The Sorption of Water on to Peptides (T. Sokoloski, et al.). DIFFERENTIAL SCANNING CALORIMETRY OF SYNTHETIC POLYMERS. Studies of Polymers and Surfactants (S. Leharne). Appendix. Index.
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