Biological specimen preparation for transmission electron microscopy
著者
書誌事項
Biological specimen preparation for transmission electron microscopy
（Practical methods in electron microscopy / edited by Audrey M. Glauert, v.17）
Portland, c1998
- hbk
- pbk
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume contains practical information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. The molecular events occurring during fixation and embedding are analyzed in detail. The reason for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.
目次
- An introduction to fixation and embedding procedures and their safe use in the laboratory
- fixatives
- fixation methods
- dehydration methods
- embedding methods
- embedding in epoxy resins
- embedding in acrylic resins
- the Lowicryl resins and embedding at low temperatures
- other embedding media
- processing schedules
- list of suppliers.
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