Lipase and phospholipase protocols
著者
書誌事項
Lipase and phospholipase protocols
（Methods in molecular biology / John M. Walker, series editor, 109）
Humana Press, 1999
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注記
Includes index
内容説明・目次
内容説明
The lipases and phospholipases represent a diverse group of enzymes that are expressed in animals, plants, fungi, and bacteria. Their ubiquitous distribution among all species is a testament to the essential roles played by these enzymes in lipid storage, mobilization and metabolism, membrane homeostasis and remodeling, endocrine and immune functions, and signal tra- duction. In humans, lipases and phospholipases are also thought to contribute to complex diseases, such as atherosclerosis, obesity, arthritis, and cancer, as well as to single gene defects, such as Wolman's disease and Type I hyperlipoproteinemia. Enzymatically, the lipases are unique, since they hydrolyze substrates that are either insoluble, or only partly soluble, in aq- ous solvents; thus, enzyme catalysis takes place at a lipid-water interface. The interface comprises at least two, and often more, discrete bulk and s- face phases, in which the enzyme, substrate, and products oflipolysis disperse among these phases based on their physical properties. Furthermore, the d- tribution of these components changes continuously as lipolysis proceeds. Thus, the lipases and phospholipases are fundamentally different from any other enzyme because of the physical complexity of the environment in which catalysis occurs.
目次
Part I. Lipase Activity, Purification, and Expression. Phospholipase A2 and Phosphatidylinositol- Specific Phospholipase C Assays by HPLC and TLC with Fluorescent Substrate, H. Stewart Hendrickson. Fluorometric Phospholipase Assays Based on Polymerized Liposome Substrates, Wonhwa Cho, Shih-Kwang Wu, Edward Yoon, and Lenka Lichtenbergova. Triglyceride Lipase Assays Based on a Novel Fluorogenic Alkyldiacyl Glycerol Substrate, Albin Hermetter. Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2, Wonhwa Cho, Sang Kyou Han, Byung-In Lee, Yana Snitko, and Rajiv Dua. Determination of Plasmalogen-Selective Phospholipase A2 Activity by Radiochemical and Fluorometric Assay Procedures, Akhlaq A. Farooqui, Hsiu-Chiung Yang, Yutaka Hirashima, and Lloyd A. Horrocks. Human Plasma Platelet-Activating Factor Acetylhydrolase, Diana M. Stafforini and Larry W. Tjoelker. Assays for Pancreatic Triglyceride Lipase and Colipase, Mark E. Lowe. Bile Salt-Activated Lipase, Chi-Sun Wang, Azar Dashti, and Deborah Downs. Determining Lipoprotein Lipase and Hepatic Lipase Activity Using Radiolabeled Substrates, Veronique Briquet-Laugier, Osnat Ben-Zeev, and Mark H. Doolittle. Lysosomal Acid Lipase: Assay and Purification, Martin Merkel, Anne-Christine Tilkorn, Heiner Greten, and Detlev Ameis. Hormone-Sensitive Lipase and Neutral Cholesteryl Ester Lipase, Cecilia Holm and Torben Osterlund. Lecithin-Cholesterol Acyltransferase: Assay of Cholesterol Esterification and Phospholipase A2 Activities, John S. Parks, Abraham K. Gebre, and James W. Furbee. Purification of Lipases and Phospholipases by Heparin-Sepharose Chromatography, Akhlaq A. Farooqui and Lloyd A. Horrocks. Large-Scale Lipoprotein Lipase Purification from Adipose Tissue, Andre Bensadoun, Jean Hsu, and Barry Hughes. Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B, Andre Bensadoun, Barry Hughes, Kristan Melford, Jean Hsu, and Dawn L. Braesaemle. High-LevelExpression and Purification of Human Hepatic Lipase from Mammalian Cells, John S. Hill. High-Level Baculoviral Expression of Hormone-Sensitive Lipase, Cecilia Holm and Juan Antonio Contreras. High-Level Baculoviral Expression of Lysosomal Acid Lipase, Anne-Christine Tilkorn, Martin Merkel, Heiner Greten, and Detlev Ameis. One-Step Purification and Biochemical Characterization of Recombinant Pancreatic Lipases Expressed in Insect Cells, Sofiane Bezzine, Francine Ferrato, Veronique Lopez, Alain de Caro, Robert Verger, and Frederic Carriere. Modulation of the Expression Level of Human Acidic Lipases by Various Signal Peptides, Stephane Canaan, Liliane Dupuis, Mireille Riviere, Robert Verger, and Catherine Wicker-Planquart. Part II. Biochemical Characterization. Immunodetection of Lipoprotein Lipase: Antibody Production, Immunoprecipitation, and Western Blotting Techniques, Mark H. Doolittle and Osnat Ben-Zeev. Immunological Characterization of Digestive Lipases, Alain De Caro, Sofiane Bezzine, Veronique Lopez, Mustapha Aoubala, Cecile Daniel, Robert Verger, and Frederic Carriere. Determining Lipase Subunit Structure by Sucrose Gradient Centrifugation, Osnat Ben-Zeev and Mark H. Doolittle. Detecting Ligands Interacting with Lipoprotein Lipase, Sivaram Pillarisetti. Monolayer Techniques for Studying Lipase Kinetics, Stephane Ransac, Margarita Ivanova, Ivan Panaiotov, and Robert Verger. Efficient Immobilization of Phospholipase A2, Wonhwa Cho and Zhen Shen. Part III. Molecular Genetic Characterization. An Introduction to Internet Resources for the Molecular and Genetic Analysis of the Lipases, Karen Reue. Techniques for the Measurement of Lipoprotein Lipase Messenger RNA, Gouri Ranganathan and Philip A. Kern. In Vitro Transcription and Translation of Lipoprotein Lipase, Gouri Ranganathan and Philip A. Kern. Induced Lipase Mutations in the Mouse, Clay F. Semenkovich. Index.
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