Cell separation : a practical approach

著者

書誌事項

Cell separation : a practical approach

edited by Derek Fisher, Gillian E. Francis, and David Rickwood

（The practical approach series, 193）

Oxford University Press, 1998

  • : hbk
  • : pbk

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注記

Includes bibliographical references and index

内容説明・目次

巻冊次

: pbk ISBN 9780199635795

内容説明

Isolating specific cells or fractionating viable cell populations is an essential step of many of the techniques used across a wide range of biological and related disciplines. This text aims to inform those working in all areas of the biological sciences of the methods which are available to them and which is likely to be the most useful. The contributing authors have provided a comprehensive guide to the methods used to prepare cell suspensions and to separate viable cells depending on their different characteristics - such as size, density, surface charge and immunological identity. Each chapter offers direct practical guidance for the various techniques and describes the advantages and limitations of each. Reproducible protocols, hints and tips for success and troubleshooting suggestions are provided.

目次

  • 1. Preparation of single cell suspensions
  • 2. Fractionation of cells by sedimentation methods
  • 3. Centrifugal elutriation
  • Appendix: Simulation of centrifugal elutriation for the Beckman E-6B chamber
  • 4. Separation and frustrationation of cells by partitioning in aqueous two-phase systems
  • Appendix: Manually operated counter current distribution apparatus
  • 5. Separation cells by flow cytometry
  • 6. Immuno methods: magnetic, columns and panning techniques
  • 7. Separation of cells using free flow electrophoresis
  • Appendix: Suppliers
巻冊次

: hbk ISBN 9780199635801

内容説明

Isolating specific cells or fractionating viable cell populations is an essential step of many of the techniques used across a wide range of biological and related disciplines. This text aims to inform those working in all areas of the biological sciences of the methods which are available to them and which is likely to be the most useful. The contributing authors have provided a comprehensive guide to the methods used to prepare cell suspensions and to separate viable cells depending on their different characteristics - such as size, density, surface charge and immunological identity. Each chapter offers direct practical guidance for the various techniques and describes the advantages and limitations of each. Reproducible protocols, hints and tips for success and troubleshooting suggestions are provided.

目次

  • Part 1 Preparation of single cell suspensions: introduction
  • methods of dispersal
  • viability measurements
  • separation of viable and non-viable cells
  • cell characterization. Part 2 Fractionation of cells by sedimentation methods: introduction
  • the theory of sedimentation
  • separation media
  • solutions for iso-osmotic gradients
  • preparation of iso-osmotic gradients
  • choice of separation method
  • separation of cells on the basis of size
  • separation of cells on the basis of density
  • conclusions. Part 3 Centrifugal elutriation: introduction
  • principles
  • equipment
  • the elutriation procedure
  • applications
  • advantages and disadvantages
  • conclusions
  • simulation of centrifugal elutriation for the Beckman JE-6B chamber. Part 4 Separation and fractionation of cells by partitioning in aqueous two-phase systems: introduction
  • theoretical background and its impact on methodology
  • practical aspects of phase partitioning
  • selected applications
  • conclusions
  • manually opeated countercurrent distribution apparatus. Part 5 Separation of cells by flow cytometry: introduction
  • the basic instrument
  • cell sorting by droplet deflection
  • other flow sorters. Part 6 Immunomethods - magnetic, column and panning techniques: introduction
  • column and panning methods
  • reagents for immunoseparation methods
  • general cell separation using immunomagnetic beads
  • functional approaches. Part 7 Separation of cells using free flow electrophoresis: introduction
  • buffers
  • preparation of cell samples for free flow electrophoresis
  • preparation and application of free flow electrophoresis for separation of whole cells
  • evaluation of the electrokinetic properties of cells separated by FFE
  • overcoming problems
  • combination of FFE with other techniques.

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